Sequences from confirmation amplicon


Confirmation sequences for line 383E09 and hit At3g09110 are shown.
Line ID 383E09
Locus At3g09110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2795153
Primers used for PCR JT05 - 8474, show primer details
   
Sequence Name 33-K027488-022-383-E09C-8409
GenBank Accession LN500858 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K027488-022-383-E09C-8409
AGCATTTACAATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGCA
TGTTGGTGTCAACATTTTACGTTTCCTTGACATGCAATGTTTCCTCAGGAAGGTGTCTGT
CAATGGAGCTTCCGAAGTGAACAAACACCCTAGCAAAGTTAGAATCTACATTTACAAAAG
AAAGTAGTGTAAGAAAGATCGTTATTAAGAAGTTGTCATCAAGGGTAGATATAAAAAGTT
CAAGTACCTCTTCAAAACCAACATCAATAAGCATTTCTTGCAGCTTATCAAAACCAGAGT
AACCAAAATCATTGAGGACATTCAAAACAAGACCCATAGAGTTCAATGCCACTTTGAGAT
CATCCGTAATAACAAATGATGTTCTGCAGCTGGGAAATACTCCATCAGCTTGCTGTTCTT
CAACCGGAATCTCACGGTGCATAGAGCTTCCACAACGACACTTCATGGTACTAACATTGC
TGAATAACTTACGGCATGCTTCAGTAGAAAC
   
Sequence Name 34-K027488-022-383-E09C-JT05
GenBank Accession LN501278 [GenBank]
Sequencing primer gene specific primer, JT05: 5'-GCCCAAATTTTGTTTCTACTGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K027488-022-383-E09C-JT05
GTACCATGAAGTGTCGTTGTGGAAGCTCTATGCACCGTGAGATTCCGCGTTGAAGAACAG
CAAGCTGATGGAGTATTTCCCAGCTGCAGAACATCATTTGTTATTACGGATGATCTCAAA
GTGGCATTGAACTCTATGGGTCTTGTTTTGAATGTCCTCAATGATTTTGGTTACTCTGGT
TTTGATAAGCTGCAAGAAATGCTTATTGATGTTGGTTTTGAAGAGGTACTTGAACTTTTT
ATATCTACCCTTGATGACAACTTCTTAATAACGATCTTTCTTACACTACTTTCTTTTGTA
AATGTAGATTCTAACTTTGCTAGGGTGTTTGTTCACTTCGGAAGCTCCATTGACAGACAC
CTTCCTGAGGAAACATTGCATGTCAAGGAAACGTAAAATGTTGACACCAACATGCAAGCT
CCACCGGGTGCAAAGCGGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11