Sequences from confirmation amplicon


Confirmation sequences for line 383G01 and hit At5g66070 are shown.
Line ID 383G01
Locus At5g66070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26421925
Primers used for PCR 78yo - 8474, show primer details
   
Sequence Name 86-K121557-0022-383-G01C-78yo
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 78yo: 5'-GGTTTCTTCTAATCTACGTTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K121557-0022-383-G01C-78yo
GGGGAAGTAGACAACCTGAAGGCGACAGAGCAAGAGACAATCTCCGGAGAGGTGTCTGTG
ATTCGGACCTTAGGGATCCTGTTGAGAGAGTCCCCAGTGAGACCCTTTGAAATGGCAGTG
TCAAAGATGTCTGTATGATCTTGGAACTGGGACTCCACAGCTCCCATCTGTTTATTATTA
ATACAAAAGTTGAGAAGGATAATGAATGATGCATCAAAGAAAAGAAATGGTAGTTGTACC
TGACTCTGGACGGCACTTAGCATTGCAGGACCGATACGCTCACGAACAAGCCTCCCGCTC
AAAAGGCTAGCAATGACATCAATCTATATATATAAAGATAAGATAGGAAAAGCATTTGAG
CATAAGAAAATAAAAGAAAATCTGGTGGTGATGATGATGAAGATTGTTACCAAGTAGAGA
AGGCATCCAATTCCAGACTCATCGGATTGCCAAAGGAGGAGGGAAGATTCAAAGACTTCG
ATGGAGAAGACGGCGCCTGAGATAGCACCAACGGCGGCTCCTCTGATGAAACCGCTCTCT
GTTTCTTGGCCGATCAAAGCCCCTGTCAATGCTCCCAGCAAAGTCCCCACTGAATCAAAT
CAACAAAGAAGTCAAATCATATTCATATCGTAATTGCAGAAACAAGGAAAATTCCTCAGG
GATCACAGAGATTGATTAATGAAGATGAATGTGAAGAAAAGAAGGAACCTAAAGCGAAGA
AGAAGGTGAAGAGAGCGGAGAAGAGGTTGGCGAGAAGGGCAGAGACGGCGAAATGGAAGG
AATCTTTAATCCGGTGGAGGACAGAGATGGGAGACAGAGAATAATAACCATCCATATCTG
CAAAAGAGATGAATTTGAAGATACAGGGAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATATAATTTTCATTCAAAATGTAG
   
Sequence Name 71-K121556-0022-383-G01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K121556-0022-383-G01C-8409
GGATGGTTATATTCTCTGTCTCCCATCTCTGTCCTCCACCGGATTAAAGATTCCTTCCAT
TTCGCCGTCTCTGCCCTTCTCGCCAACCTCTTCTCCGCTCTCTTCACCTTCTTCTTCGCT
TTAGGTTCCTTCTTTTCTTCACATTCATCTTCATTAATCAATCTCTGTGATCCCTGAGGA
ATTTTCCTTGTTTCTGCAATTACGATATGAATATGATTTGACTTCTTTGTTGATTTGATT
CAGTGGGGACTTTGCTGGGAGCATTGACAGGGGCTTTGATCGGCCAAGAAACAGAGAGCG
GTTTCATCAGAGGAGCCGCCGTTGGTGCTATCTCAGGCGCCGTCTTCTCCATCGAAGTCT
TTGAATCTTCCCTCCTCCTTTGGCAATCCGATGAGTCTGGAATTGGATGCCTTCTCTACT
TGGTAACAATCTTCATCATCATCACCACCAGATTTTCTTTTATTTTCTTATGCTCAAATG
CTTTTCCTATCTTATCTTTATATATATAGATTGATGTCATTGCTAGCCTTTTGAGCGGGA
GGCTTGTTCGTGAGCGTATCGGTCCTGCAATGCTAAGTGCCGTCCAGAGTCAGGTACAAC
TACCATTTCTTTTCTTTGATGCATCATTCATTATCCTTCTCAACTTTTGTATTAATAATA
AACAGATGGGAGCTGTGGAGTCCCAGTTCCAAGATCATACAGACATCTTTGACACTGCCA
TTTCAAAGGGTCTCACTGGGGACTCTCTCAACAGGATCCCTAAGGTCCGAATCACAGACA
CCTCTCCGGAGATTGTCTCTTGCTCTGTCTGCCTTCAGGTTTGTCTCACTTCCCCCCTCT
GCTTTTGAACATCATCTTCCGCGGAACGTAGATAAGAAAAAAACCATTTT

Last Updated on 06.12.2018 16:11