Sequences from confirmation amplicon


Confirmation sequences for line 383H09 and hit At4g18290 are shown.
Line ID 383H09
Locus At4g18290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10117736
Primers used for PCR 29kh - 8474, show primer details
   
Sequence Name 75-K113549-0022-383-H09C-29kh
GenBank Accession LN513276 [GenBank]
Sequencing primer gene specific primer, 29kh: 5'-TTGTGCAGGATTTTACTGTCTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K113549-0022-383-H09C-29kh
CATATATTCCCAAAAGAACTTCACTGTAGAACTCTTAGCTGAAAAAAGAGAAATGACTGT
AATCCTCACATTCATGTAGGTTCAAGGCAAGGCGGTTGTTGGCGATGCATTTGGAGAGAT
TGGTGTGTTATGCTATACACCACAACCTTTCACAGTTAGGACAACAGAACTATCTCAGAT
ACTACGGATAAGCAAAAAATCTCTAATGAGTGCTATGCGAGCTCATGTCGAAGATGGACG
AGTCATAATGAACAACCTCTTCATGGTAAGTAAGAATGATATAACATCCTGGGAACTAAT
TTTATTTCAGAGATGTTCAATTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 10-K113548w-0022-383-H09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K113548w-0022-383-H09C-8409
TCGGATGAGCATTTCATTGAATATATTCAATTGAACATCTCTGAAATAAAATTAGTTCCC
AGGATGTTATATCATTCTTACTTACCATGAAGAGGTTGTTCATTATGACTCGTCCATCTT
CGACATGAGCTCGCATAGCACTCATTAGAGATTTTTTGCTTATCCGTAGTATCTGAGATA
GTTCTGTTGTCCTAACTGTGAAAGGTTGTGGTGTATAGCATAACACACCAATCTCTCCAA
ATGCATCGCCAACAACCGCCTTGCCTTGAACCTACATGAATGTGAGGATTACAGTCATTT
CTCTTTTTTCAGCTAAGAGTTCTACAGTGAAGTTCTTTTGGTGATATATATTGTTCACAT
ACCTGATCTTCTTCGCCAACGTAGACNGTAAAATCCTGCACA

Last Updated on 06.12.2018 16:11