Sequences from confirmation amplicon


Confirmation sequences for line 384C12 and hit At3g14150 are shown.
Line ID 384C12
Locus At3g14150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4690699
Primers used for PCR TK30 - 8474, show primer details
   
Sequence Name 12-K103674-0022-384-C12C-TK30
GenBank Accession LN491542 [GenBank]
Sequencing primer gene specific primer, TK30: 5'-TCTTGTATGGTGATCAGAAGGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103674-0022-384-C12C-TK30
GAGATGTTCTATATTCGGTGAAAGATCAAGAAATGTCTTCCATGGCGTCAGATCAATTCG
TAAGGATACTGCCCCCAAGGCTCTTCTTCAGGAAACAAATCTTCAACCAGGTCGTCTGAA
TTTAACTTTTAGGCAGTACTAGAGATAGGATCAAACTCATGATTGATGCTGCAGCTCTTT
CCTTTATATTTATATTTTATGCACTAACCACATCATTGTGTCAGACAAGCATTCAATATG
TTTATCATATGTACTTTTCAAAAGATGAGTGTTGAATTGAATCCATGCTTCAGTCCTTGT
TCTTCTAACATGGCTGCTGGTTGCCACGGTTCTTCAGCTGTCTGATTAAGTTTCTCAGAC
CATATCTGTTGGTTTTTTGGATCAGAGCATAGAGTGAAGTCTCTCATTCTCTGTTAGGCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCG
   
Sequence Name 28-K103675-0022-384-C12C-8409
GenBank Accession LN498808 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K103675-0022-384-C12C-8409
ATTCGGACATGAGCCATTTACAATTGAATATATCCTGCCTAACAGAGAATGAGAGACTTC
ACTCTATGCTCTGATCCAAAAAACCAACAGATATGGTCTGAGAAACTTAATCAGACAGCT
GAAGAACCGTGGCAACCAGCAGCCATGTTAGAAGAACAAGGACTGAAGCATGGATTCAAT
TCAACACTCATCTTTTGAAAAGTACATATGATAAACATATTGAATGCTTGTCTGACACAA
TGATGTGGTTAGTGCATAAAATATAAATATAAAGGAAAGAGCTGCAGCATCAATCATGAG
TTTGATCCTATCTCTAGTACTGCCTAAAAGTTAAATTCAGACGACCTGGTTGAAGATTTG
TTTCCTGAAGAAGAGCCTTGGGGGCAGTATCCTTACGAATTGATCTGACGCCATGGAAGA
CATTTCTTGATCTTTCACCGAATATTAGAACATCTCCAGACTCCAAGATTAACGTGTCTG
CCTTGTCAACATCCTTCTGATCACCATACAAG

Last Updated on 06.12.2018 16:11