Sequences from confirmation amplicon


Confirmation sequences for line 384F02 and hit At5g25060 are shown.
Line ID 384F02
Locus At5g25060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8639448
Primers used for PCR AP12 - 8474, show primer details
   
Sequence Name 06-K106147-0022-384-F02C-AP12
GenBank Accession LN488821 [GenBank]
Sequencing primer gene specific primer, AP12: 5'-GAAATTTGTTCATCGGAGATTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K106147-0022-384-F02C-AP12
TCGCAGAAGCAGAGGATGAAACGGCTCGTTTGTATCAAGAATTCGTGGAATCGTTTCAAG
GGGATAATGCAACTACTAAGACTTTTGTTCGAGGTGGGACGATAAATCCTGGTGACAAGC
CTAAGGTTGATTCTGAAGGTTAGTCTTTGCAAAGGCTACACAGCTTTTTAACTGTTTGTT
TACAATTGCTTCTCTCTTTGTGTGTACTTTGTTCTTTTTGATTGGTGTCAAATGGTTAGA
GCAATGCTTTGATTATATTGGGAACGCATTAATAAGTGTATGGACCTTTTTTCTATGCGT
TCGCGGATGGCAGGTGAAAAGTCCAAAGATGGGGGTTCGGTTTCAAAGAAGGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCA
   
Sequence Name 22-K106148-0022-384-F02C-8409
GenBank Accession LN496122 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K106148-0022-384-F02C-8409
TCGGACTGAGCCATTTACATTGAATATATCCCTTCTTTGAACCGAACCCCCATCTTTGGA
CTTTTCACCTGCCATCCGCGAACGCATAGAAAAAAGGTCCATACACTTATTAATGCGTTC
CCAATATAATCAAAGCATTGCTCTAACCATTTGACACCAATCAAAAAGAACAAAGTACAC
ACAAAGAGAGAAGCAATTGTAAACAAACAGTTAAAAAGCTGTGTAGCCTTTGCAAAGACT
AACCTTCAGAATCAACCTTAGGCTTGTCACCAGGATTTATCGTCCCACCTCGAACAAAAG
TCTTAGTAGTTGCATTATCCCCTTGAAACGATTCCACGAATTCTTGATACAAACGAGCCG
TTTCATCCTCTGCTTTCTGCGATTAAGATCGAAAACCCCATCGATTAATCAATCAAATCA
ATCTCCGATAACAAAATTT

Last Updated on 06.12.2018 16:11