Sequences from confirmation amplicon


Confirmation sequences for line 385C05 and hit At4g05410 are shown.
Line ID 385C05
Locus At4g05410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2744291
Primers used for PCR 03mm - 8474, show primer details
   
Sequence Name 54-K114597-0022-385-C05C-03mm
GenBank Accession LN508559 [GenBank]
Sequencing primer gene specific primer, 03mm: 5'-CTGCAACGAAACAAAAGTGTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K114597-0022-385-C05C-03mm
CCACTGCACAGAGATGAAAGGGGAGGATCTAAATTAGGTTTCACCTAAGATTCCATTGGT
GGTAATCCCATCAGGTATATCTTGATGTGCATTCTTGAAAACGAACACCGNGTTTCTTTT
TTAACATGCCCCAAAGAGCAACAGTTCCATTATCCGAACCAGACAGGTACTCATTATCAC
TGATGAAACAGCAGCTCTCAAGAGACGATGCAGGAGCTCGATAGATCATACGGGTGCTCT
CAGGCACCTATAATCATATAACAAGACTTACAAATACAGAATAATAATAGAAACCTTATG
ATGATATCTTCCAACAGTGTTTACATGGAGGATAGAACTATTACCTTATGATACAGCATG
GTTCTATCTCTTCCAACCGTAAGTGCACGCTCCTTCCTCAGTGCATCAATGGCTTATGAT
TTTCAATTGTAGAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TG
   
Sequence Name 14-K114596-0022-385-C05C-8409
GenBank Accession LN492609 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114596-0022-385-C05C-8409
AATCATAAGCCATTGATGCACTGAGGAAGGAGCGTGCACTTACGGTTGGAAGAGATAGAA
CCATGCTGTATCATAAGGTAATAGTTCTATCCTCCATGTAAACACTGTTGGAAGATATCA
TCATAAGGTTTCTATTATTATTCTGTATTTGTAAGTCTTGTTATATGATTATAGGTGCCT
GAGAGCACCCGTATGATCTATCGAGCTCCTGCATCGTCTCTTGAGAGCTGCTGTTTCATC
AGTGATAATGAGTACCTGTCTGGTTCGGATAATGGAACTGTTGCTCTTTGGGGCATGTTA
AAAAAGAAACCGGTGTTCGTTTTCAAGAATGCACATCAAGATATACCTGATGGGATTACC
ACCAATGGAATCTTAGGTGAAACCTAATTTAGATCCTCCCCATTTCATCTCTGTTGCAGT
TGGATCTTGACATTGCAGTATATAGTTATAAAACACT

Last Updated on 06.12.2018 16:11