Sequences from confirmation amplicon


Confirmation sequences for line 386A11 and hit At3g01290 are shown.
Line ID 386A11
Locus At3g01290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:88266
Primers used for PCR 03kx - 8474, show primer details
   
Sequence Name 84-K115459-0022-386-A11C-03kx
GenBank Accession LN514465 [GenBank]
Sequencing primer gene specific primer, 03kx: 5'-GTTACGAAATCCTTCAAACCCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K115459-0022-386-A11C-03kx
TAACGTGCCAGNAACGAAATCACGCCGGTAAGTCCTCAAAATCGTAAAACCCTAAATCAA
AATCTAAACAAATAAATGGTCAAAACCCTGAATTCAATTGTTGTTACAGCGGCGAGGATG
AGAGTGGCAGCGAGCGAAAAAGCAGAGGCTGAGAAAATCATTCAGATCAAAAGAGCAGAG
GGTGAAGCAGAGTCAAAGTACCTGTCGGGACTCGGAATCGCTCGGCAGAGACAAGCGATC
GTGGACGGTCTGAGAGACAGTGTTCTAGGGTTCGCAGGAAACGTGCCAGGGACGTCAGCG
AAGGATGTGTTGGACATGGTGATGATGACTCAGTACTTTGACACAATGAGAGATATCGGA
GCAACTTCTAAATCCTCTGCGGTGTTTATCCCTCACGGTCCAGGCGCCGTCTCTGACGTG
GCAGCGCAGATTCGAAATGGATTATTACAGGCCATCATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 35-K115458-0022-386-A11C-8409
GenBank Accession LN501950 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K115458-0022-386-A11C-8409
TCATTGAATATGATGGCCTGTAATAATCCATTTCGAATCTGCGCTGCCACGTCAGAGACG
GCGCCTGGACCGTGAGGGATAAACACCGCAGAGGATTTAGAAGTTGCTCCGATATCTCTC
ATTGTGTCAAAGTACTGAGTCATCATCACCATGTCCAACACATCCTTCGCTGACGTCCCT
GGCACGTTTCCTGCGAACCCTAGAACACTGTCTCTCAGACCGTCCACGATCGCTTGTCTC
TGCCGAGCGATTCCGAGTCCCGACAGGTACTTTGACTCTGCTTCACCCTCTGCTCTTTTG
ATCTGAATGATTTTCTCAGCCTCTGCTTTTTCGCTCGCTGCCACTCTCATCCTCGCCGCT
GTAACAACAATTGAATTCAGGGTTTTGACCATTTATTTGTTTAGATTTTGATTTAGGGTT
TTACGATTTTGAGGACTTACCGGCGTTGATTTCGTTCATGGCACGTTTAACCTGTTGATC
AGGCTCAATGTCGATAATTAGGGTTTGAAGGATTTCGTAA

Last Updated on 06.12.2018 16:11