Sequences from confirmation amplicon


Confirmation sequences for line 386C07 and hit At3g59380 are shown.
Line ID 386C07
Locus At3g59380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21945608
Primers used for PCR IF32 - 8474, show primer details
   
Sequence Name 60-K101385-0022-386-C07C-8409
GenBank Accession LN510209 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K101385-0022-386-C07C-8409
ATAGAAGATCCAAAAGGGTGCTCAGAGCGAATCCATGGAAGCAATCTGTGCGGGATAGAA
CATTCAAACAGACTGAGGAAACACTTGGATCACTAATCCAGGATTCTTTGTCGTCTTTGT
AAAGAGCTTTTAGGTATCGCCATGAGCTCTCGTTTGCAGGATTGGTTAAAATGGCTTTGA
TTGTGTAGCTTACTTCAGATTCTCTCATGGCTTCTAGGCCTCCCAACAAAGGAGATTGGG
TGATGACATAATACCTCTGTCAGATTAAGGGAATGAGATTATACAAATAGGTGACAGAAT
CGGCAATTGATAGATTAGAGATACAGAATTTCGTATGTGTATCAACGATCAAGTTACCTG
ATTCCAGGCGGAATTGTTAAAGACGTCAGCT
   
Sequence Name 60-K101391-0022-386-C07C-IF32
GenBank Accession LN510210 [GenBank]
Sequencing primer gene specific primer, IF32: 5'-GAAGCTGACGTCTTTAACAATTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K101391-0022-386-C07C-IF32
ATACGAAATTCTGTATCTCTAATCTATCAATTGCCGATTCCGTCAACTATTTGTATAATC
TCATTCCCTTAATCTGACAGAGGTATTATGTCATCACCCAATCTCCTTTGTTGGGAGGCC
TAGAAGCCATGAGAGAATCTGAAGTAAGCTACACAATCAAAGCCATTTTAACCAATCCTG
CAAACGAGAGCTCATGGCGATACCTAAAAGCTCTTTACAAAGACGACAAAGAATCCTGGA
TTAGTGATCCAAGTGTTTCCTCAGTCTGTTTGAATGTTCTATCCCGCACAGATTGCTTCC
ATGGATTCGCTCTGAGCACCCTTTTGGATCTTCTATGTGATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTTCAAAATGTAGA

Last Updated on 06.12.2018 16:11