Genotyping and primer details

Genotyping and primer details for 386C07 - At3g59380
Line ID 386C07
Locus At3g59380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21945608 of line 386C07 Chr3:21945608
Insertion allele amplicon 8474 - IF32
Locus specific primer IF32: 5'-GAAGCTGACGTCTTTAACAATTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21945216
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11