Sequences from confirmation amplicon


Confirmation sequences for line 386D01 and hit At1g35230 are shown.
Line ID 386D01
Locus At1g35230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12917361
Primers used for PCR 43ns - 8474, show primer details
   
Sequence Name 20-K114963-0022-386-D01C-43ns
GenBank Accession LN495333 [GenBank]
Sequencing primer gene specific primer, 43ns: 5'-ATTCAAGAATGCCAATAATCAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114963-0022-386-D01C-43ns
AATGTACAATATACACCAACATAACACGATAATAAAGCATCATATAAAACACTATAAACT
ACATAATTAAATAGTAACAAAAGGACAAACAATGAACCCAAAAATAATTCCTAGTGAATC
TATTCGATGGGTCAATAGCCTCTACAGCAACAAAGCGGCGATCACGGCCACTCCGGCGAA
AGTTCCGACGAGGGAGACTCTGCTAACTGCAGCTGCGTTTGGAGAGCCACTTAGGGGAGA
CTGAGCAGGTCCAGCTTCTCCAGACGGAACATTTGTGCCCGGAGCTCCGGATGGGGAAGT
AGACGTCGGAGGAGCTGGTGGAGATTCAGTAGGCGGCTGACTAACCGGAGGAGCAGTGGT
AGGAGCCGAAAGATACCAAATTAGTCGAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 04-K114962-0022-386-D01C-8409
GenBank Accession LN488028 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114962-0022-386-D01C-8409
GTATCTTTCGGCTCCTACACTGCTCCTCCGGTTAGTCAGCCGCCTACTGAATCTCCACCA
GCTCCTCCGACGTCTACTTCCCCATCCGGAGCTCCGGGCACAAATGTTCCGTCTGGAGAA
GCTGGACCTGCTCAGTCTCCCCTAAGTGGCTCTCCAAACGCAGCTGCAGTTAGCAGAGTC
TCCCTCGTCGGAACTTTCGCCGGAGTGGCCGTGATCGCCGCTTTGTTGCTGTAGAGGCTA
TTGACCCATCGAATAGATTCACTAGGAATTATTTTTGGGTTCATTGTTTGTCCTTTTGTT
ACTATTTAATTATGTAGTTTATAGTGTTTTATATGATGCTTTATTATCGTGTTATTGTTG
GTGTTATATTTGTTACAATTTTTTTATAGTTTCCTTGATTATTGGCNTTCTTGAA

Last Updated on 06.12.2018 16:11