Sequences from confirmation amplicon


Confirmation sequences for line 386E04 and hit At4g02160 are shown.
Line ID 386E04
Locus At4g02160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:954943
Primers used for PCR 53ra - 8474, show primer details
   
Sequence Name 30-K115914-0022-386-E04C-53ra
GenBank Accession LN499861 [GenBank]
Sequencing primer gene specific primer, 53ra: 5'-AACGTCATAAACCTAATGCCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K115914-0022-386-E04C-53ra
ACCGATTCATTTACTCTCCACATCATAAACTTCTAATCTCTCTTCTTTAGCTTCCATGTC
TCTGATTTTCTCTTCAAGCAAATTGGATTCTCAAACCAATAGGAAAATTCTAAATTATAT
TGGATTGTACCCATAAGTGAATTATAAACTTCTACTTAACTCATTATTGGGTTTTTGGTT
CAAAAGAAAAAAAAGAAAAAAACGAATTGCTTGAAATATGACAACAACAAAGGCTTGTAG
CTCAGAGTCATGTATATGACACCCTAGGAAAACTGCCAAAGAAGCTCAGAGTCATGTATA
TAATTTGCGTACAAATTCATCATCTACTATATCACTCACAAGAAGCTAGCTGCAATTTGG
TTCTAGCATTATGACTTGAAGATGATATATAGTAAACCAAAATGACATCAGTTTTATAAA
TATATTTTAATATAGTTTGCGTACAAATTCATCGTCTACTATAAAAGTATACAATCAAGA
CTAACTGAATACAATATATATATCATTACTTAAATCATCAATATGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTC
   
Sequence Name 04-K115913-0022-386-E04C-8409
GenBank Accession LN488057 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115913-0022-386-E04C-8409
ATTCAGTTAGTCTTGATCGTATACTTTTATAGTAGACGATGAATTTGTACGCAAACTATA
TTAAAATATATTTATAAAACTGATGTCATTTTGGTTTACTATATATCATCTTCAAGTCAT
AATGCTAGAACCAAATTGCAGCTAGCTTCTTGTGAGTGATATAGTAGATGATGAATTTGT
ACGCAAATTATATACATGACTCTGAGCTTCTTTGGCAGTTTTCCTAGGGTGTCATATACA
TGACTCTGAGCTACAAGCCTTTGTTGTTGTCATATTTCAAGCAATTCGTTTTTTTCTTTT
TTTTCTTTTGAACCAAAAACCCAATAATGAGTTAAGTAGAAGTTTATAATTCACTTATGG
GTACAATCCAATATAATTTAGAATTTTCCTATTGGTTTGAGAATCCAATTTGCTTGAAGA
GAAAATCAGAGACATGGAAGCTAAAGAAGAGAGATTAGAAGTTTATGATGTGGAGAGTAA
ATTGAATCGGTTGCAATTCGGTTTATTATACTAGATTTTTGGTTTGGCATT

Last Updated on 06.12.2018 16:11