Sequences from confirmation amplicon


Confirmation sequences for line 386F12 and hit At1g29350 are shown.
Line ID 386F12
Locus At1g29350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10271618
Primers used for PCR 17qe - 8474, show primer details
   
Sequence Name 25-K115349-0022-386-F12C-17qe
GenBank Accession LN497583 [GenBank]
Sequencing primer gene specific primer, 17qe: 5'-TATAGTATAGTTTTATGACAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115349-0022-386-F12C-17qe
CCCCCAGCAGAACATAATCCCATCTGTATTCACATGATCAATAAACACAAGCCCACTCCA
AAATGAAACAGGAAAAAGAATTTGAACATAATGGCACACACTAGCAATAAATGGGAAACA
TTTTATAAAGATTCAAAATATTACCTTTGTGGTGCTCTACTGGATGGCTCTGTGTCTGAT
ATGGATTATCATCAATTTCTGACGAAACTACAGATTCATCTTCTTGAACAGAGTCGGCTT
GCACATGATCGACCCCACGACTTTCAGACGGGCCACTTTCTGCTATATGAATATTTTCTA
ACCGGTCCTTCAGATATTGATCTCCTCTATCGGATTGGAAATCAGCTAGACCATTGCGTA
CTTCTGATTCTGCTGGTGCTACTGATAAAGAAGATGTACTAGGAGCCAGCGGCTTCTCAA
TCGACGGCCATTCATCTTTAACAGGGGCCTGCTGATTTGCATTAACTTCGTGTTCATGAT
TTATCTCAGAACCCACATTGACATTTTGCTTTGTTGTCTTGTTCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 09-K115348-0022-386-F12C-8409
GenBank Accession LN490317 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115348-0022-386-F12C-8409
TCGGACTGAGCCTTTACATTGAATATATCCTGAACAGACAACAAAGCAAAATGTCAATGT
GGGTTCTGAGATAAATCATGAACACGAAGTTAATGCAAATCAGCAGGCCCCTGTTAAAGA
TGAATGGCCGTCGATTGAGAAGCCGCTGGCTCCTAGTACATCTTCTTTATCAGTAGCACC
AGCAGAATCAGAAGTACGCAATGGTCTAGCTGATTTCCAATCCGATAGAGGAGATCAATA
TCTGAAGGACCGGTTAGAAAATATTCATATAGCAGAAAGTGGCCCGTCTGAAAGTCGTGG
GGTCGATCATGTGCAAGCCGACTCTGTTCAAGAAGATGAATCTGTAGTTTCGTCAGAAAT
TGATGATAATCCATATCAGACACAGAGCCATCCAGTAGAGCACCACAAAGGTAATATTTT
GAATCTTTATAAAATGTTTCCCATTTATTGCTAGTGTGTGCCATTATGTTCAAATTCTTT
TTCCTGTTTCATTTTGGAGTGGGCTTGTGTTTATTGATCATGTGAATACAGATGGGATTA
TAGTTCTGCTGGGGGGTAATGAAAGCTTCTTTGCTGTTTTGTTTGTCATAAAANATTACT
AT

Last Updated on 06.12.2018 16:11