Sequences from confirmation amplicon


Confirmation sequences for line 386H06 and hit At4g09340 are shown.
Line ID 386H06
Locus At4g09340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5926860
Primers used for PCR MN04 - 8474, show primer details
   
Sequence Name 25-K028159-022-386-H06C-8409
GenBank Accession LN497302 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K028159-022-386-H06C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTTACAGTTC
TGACATATCTTTGTTATGAGATGCCAGTGTAAATGGAACTCTGGTAGGGACAGTCTCCAA
GTACATCAAGGGTCCTCTATTCCCTACTGTAGCTGTACATAGCCAAAACGAAGAGTATGC
TCTCTTTGTTTCTTAATATGATGTCAGCGTGAACTATTTTTCAAGTGCTTGAATATGTTC
TTTGTTTGATGTTCTGAATCTTGGTAGGGTTACAGTCAACTTCGGGCAGGAGAAGTTTGC
CTTTGATTTTAAGGTAAGATCCTTGAATATTTTTGGTATACTAGTGGAGTTTCTAGCTCT
TAGCTTGTGTAATTTATCGATGGGA
   
Sequence Name 26-K028159-022-386-H06C-MN04
GenBank Accession LN497778 [GenBank]
Sequencing primer gene specific primer, MN04: 5'-ATCCCATCGATAAATTACACAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K028159-022-386-H06C-MN04
AAGAGCTAGAAACTCCACTAGTATACCAAAAATATTCAAGGATCTTACCTTAAAATCAAA
GGCAAACTTCTCCTGCCCGAAGTTGACTGTAACCCTACCAAGATTCAGAACATCAAACAA
AGAACATATTCAAGCACTTGAAAAATAGTTCACGCTGACATCATATTAAGAAACAAAGAG
AGCATACTCTTCGTTTTGGCTATGTACAGCTACAGTAGGGAATAGAGGACCCTTGATGTA
CTTGGAGACTGTCCCTACCAGAGTTCCATTTACACTGGCATCTCATAACAAAGATATGTC
AGAACTGTAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11