Sequences from confirmation amplicon


Confirmation sequences for line 388B09 and hit At4g37900 are shown.
Line ID 388B09
Locus At4g37900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17822374
Primers used for PCR MO10 - 8474, show primer details
   
Sequence Name 57-K023370w-022-388-B09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K023370w-022-388-B09C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTACATCCTTAT
GGTAAAGCTGTGGGGTTACTCGATTTGAAGTTCGGTTCTATTGAGGTAACGTTTGCAACT
TTGATCTTGTAATCAACCATTTTGTTATTTCTGACTTGGGGTTTGTTTCAATATGTAGGC
AAATGAGAAATGGCTGGTTTTGCCTGGAATGGTATCTTCTTTCATACTCAGTGATCTTCT
TAAGAAGGAAGGCTTTAGTGCAGCCGCAAAAGACACGGTGAAAGCTAATGGAATAACAGA
GGAAAGTACGGAGATTGATGTTTTGAGTCAAGAAAAGCTAGAAGAAGAAACAATGATGGA
TGTGGATACGACTACACCAGTTGCTGTAGCGGCCGAGAAGATCAACGGTGGTGCTAGGTG
CTTTTCGAAGGAGCTAAGTGGGAACATGATTGAAGAAGAAGGTGGTCACTGTGGTGGTTG
TGGAGGTTGTGGTGGATGTGGAGG
   
Sequence Name 61-K023370w-022-388-B09C-MO10
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, MO10: 5'-TCATCATATTACCACATCCTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K023370w-022-388-B09C-MO10
CGCAATTCCGCAGCCAGTGGAACCGCCAGTGCAGGATCCGCCGCCGCAGCCTTCTATCTT
TGTCATCCCACCGCACCTACCACCACCACCACATCCACCTCCACCTCCACATCCACCACA
ACCTCCACAACCACCACAGTGACCACCTTCTTCTTCAATCATGTTCCCACTTAGCTCCTT
CGAAAAGCACCTAGCACCACCGTTGATCTTCTCGGCCGCTACAGCAACTGGTGTAGTCGT
ATCCACATCCATCATTGTTTCTTCTTCTAGCTTTTCTTGACTCAAAACATCAATCTCCGT
ACTTTCCTCTGTTATTCCATTAGCTTTCACCGTGTCTTTTGCGGCTGCACTAAAGCCTTC
CTTCTTAAGAAGATCACTGAGTATGAAAGAAGATACCATTCCAGGCAAAACCAGCCATTT
CTCATTTGCCTACATATTGAAACAAACCCCAAGTCAGAAATAACAAAATGGTTGATTACA
AGATCAAAGTTGCAAACGTTACCTCAATAGAACCGAACTTCAAATCGAGTAACCCCACAG
CTTTACCATAAGGATGTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATG

Last Updated on 06.12.2018 16:11