Sequences from confirmation amplicon


Confirmation sequences for line 388E02 and hit At5g65670 are shown.
Line ID 388E02
Locus At5g65670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26254518
Primers used for PCR 02rh - 8474, show primer details
   
Sequence Name 09-K115882-0022-388-E02C-02rh
GenBank Accession LN490335 [GenBank]
Sequencing primer gene specific primer, 02rh: 5'-GACTGTCCTTTGGGTATGTTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115882-0022-388-E02C-02rh
GACATCCAGTTTTTCTCAGTATACACTGAACTTTTAGCTTCAGCAAACTGATCCATTGTG
TCAGAAAAGCCTCTTTTGTTTCCCGATGCATTGTTCTTTTGCGAGGAGGAGCATATCTCA
TCTTTAGAAGGAAGCAAAGGAAAGAATGGCTTCTCATCTAGCTTTGCTGGGCTCAAAAGG
TTAAGCTCTGTGTCTCTCGCAGGAGATTGTGATCCAGGAAGACCAAGTGTCAGCTCCGTA
GCCTTGAGGCTGATAGTAGCTTTGTCATCTTCAGCAAGGGGAGAGAGAGTAGAGCTTCCA
ACAGAAGAACAATCAGAGAGACCCAAGTAGTTATGCTCTTTAAGTCCTCCTAGAGTGTTC
CTGGAGATGCAATTGCTAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGA
   
Sequence Name 01-K115881-0022-388-E02C-8409
GenBank Accession LN486714 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K115881-0022-388-E02C-8409
GCATTTCTAGCATTGCATCTCCAGGACACTCTAGGAGGACTTAAAGAGCATAACTACTTG
GGTCTCTCTGATTGTTCTTCTGTTGGAAGCTCTACTCTCTCTCCCCTTGCTGAAGATGAC
AAAGCTACTATCAGCCTCAAGGCTACGGAGCTGACACTTGGTCTTCCTGGATCACAATCT
CCTGCGAGAGACACAGAGCTTAACCTTTTGAGCCCAGCAAAGCTAGATGAGAAGCCATTC
TTTCCTTTGCTTCCTTCTAAAGATGAGATATGCTCCTCCTCGCAAAAGAACAATGCATCG
GGAAACAAAAGAGGCTTTTCTGACACAATGGATCAGTTTGCTGAAGCTAAAAGTTCAGTG
TATACTGAGAAAAACTGGATGTTCCCTGAAGCAGCAGCCACCCAGTCTGTAACAAAGAAA
GATGTGCCACAAAACATACCCAAGGGACAGT

Last Updated on 06.12.2018 16:11