Sequences from confirmation amplicon


Confirmation sequences for line 388H10 and hit At5g04220 are shown.
Line ID 388H10
Locus At5g04220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1156376
Primers used for PCR 25aq - 8474, show primer details
   
Sequence Name 76-K110174-0022-388-H10C-25aq
GenBank Accession LN513416 [GenBank]
Sequencing primer gene specific primer, 25aq: 5'-GAATGGAACGAACACTTTAAGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K110174-0022-388-H10C-25aq
AGTCCTTCACTAGAAGTCTTTGACTGGGACAAGGTCCATGCATCTAATGATCCCATTTCT
TCTCTAGTTCAACATTTTATGCCATTCTTACTAAAGCGGCTCTGTATCTGTATAGGTCGG
TGGGCACGACAGATTAGGAATGCAGATGATCCCTTTGCAAAAGATTAACCCGGGTGAGAG
GAAGGAGTTTAATCTTGATCTGATTAAGAACTCAAATGTTGTTATGGATTCTGGAGACAA
GAAAAAAAGAGGAAGATTAGAGGTTGATCTCAGGTATGTGCCATTCAGAGAAGAAAGCAT
TAAGCGTAGAAAAGAGAGCAGAGAAGAGAAATCATCAGAGGACGATGATTTTCTCAGCCA
AGCCGGTCTACTCTCGGTTGCAGTCCAATCTGCAAAAGATGTCGAGGGCAAGAAGAAACA
CTCGAACCCTTATGCTGTTGTTCTCTTCAGAGGAGAAAAGAAGAGAACTAAGGTAAAATT
CTAAATCTGCATTTTGCTGAGAAGCTACTTAAGAACTATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCA
   
Sequence Name 66-K110173-0022-388-H10C-8409
GenBank Accession LN511634 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K110173-0022-388-H10C-8409
GCTTCTCAGCAAATGCAGATTTAGAATTTTACCTTAGTTCTCTTCTTTTCTCCTCTGAAG
AGAACAACAGCATAAGGGTTCGAGTGTTTCTTCTTGCCCTCGACATCTTTTGCAGATTGG
ACTGCAACCGAGAGTAGACCGGCTTGGCTGAGAAAATCATCGTCCTCTGATGATTTCTCT
TCTCTGCTCTCTTTTCTACGCTTAATGCTTTCTTCTCTGAATGGCACATACCTGAGATCA
ACCTCTAATCTTCCTCTTTTTTTCTTGTCTCCAGAATCCATAACAACATTTGAGTTCTTA
ATCAGATCAAGATTAAACTCCTTCCTCTCACCCGGGTTAATCTTTTGCAAAGGGATCATC
TGCATTCCTAATCTGTCGTGCCCACCGACCTATACAGATACAGAGCCGCTTTAGTAAGAA
TGGCATAAAATGTTGAACTAGAGAAGAAATGGGATCATTAGATGCATGGACCTTGTCCCA
GTCAAAGACTTCTAGTTGAAGGACTTGTGAGTTTGGGTCCTTGACGATGAGCTTAAAGTG
TTCGTTC

Last Updated on 06.12.2018 16:11