Sequences from confirmation amplicon


Confirmation sequences for line 389A02 and hit At1g34575 are shown.
Line ID 389A02
Locus At1g34575 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12657912
Primers used for PCR KP64 - 8474, show primer details
   
Sequence Name 47-K034066-022-389-A02C-KP64
GenBank Accession LN506120 [GenBank]
Sequencing primer gene specific primer, KP64: 5'-TTAATCTACGTGCAATTGAGATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K034066-022-389-A02C-KP64
TCCGGTGCAACCCTNAGGAGAGATCTACTACAACGTTGCCAATAAAAGCAACAACCTCCG
TGGTTTCCCCGCTGGAATCTGTCCCGGTCTCGGTGCTGGTGGGCATTTTAGCGGTGGAGG
TTACGGAAACATGATGAGGAAATACGGTTTATCGATTGATAACATTATAGACGCTAAGAT
TGTTGATGCTAACGCAAGAGTCTTGGACCGGAGTTCCATGGGAGAAGATCTTTTCTGGGC
CTTACGAGGCGGTGGAGCCGCTAGCTTCTGTGTTGTCTTAGCTTGGAAGATCAAGCTAGT
TCCTGTCCCGGAGAAAGTTACAGTCTTTAACGTTGAAACAATCGGAAACAGAGGAGTAAT
CCCGACAAGATGAGCAATTACGCGCAGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTC
   
Sequence Name 31-K034066-022-389-A02C-8409
GenBank Accession LN500063 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K034066-022-389-A02C-8409
TGCGCGTAAATTGCTCATCTTGTCGGGATTACTCCTCTGTTTCCGATTGTTTCAACGTTA
AAGACTGTAACTTTCTCCGGGACAGGAACTAGCTTGATCTTCCAAGCTAAGACAACACAG
AAGCTAGCGGCTCCACCGCCTCGTAAGGCCCAGAAAAGATCTTCTCCCATGGAACTCCGG
TCCAAGACTCTTGCGTTAGCATCAACAATCTTAGCGTCTATAATGTTATCAATCGATAAA
CCGTATTTCCTCATCATGTTTCCGTAACCTCCACCGCTAAAATGCCCACCAGCACCGAGA
CCGGGACAGATTCCAGCGGGGAAACCACGGAGGTTGTTGCTTTTATTGGCAACGTTGTAG
TAGATCTCTCCTAGGGTTGCACCGGATTGTACCCACGCGGTATCTAGTTTTGGATCGATC
TCAATT
Primers used for PCR 44wr - 8474, show primer details
   
Sequence Name 66-K118656-0022-389-A02C-44wr
GenBank Accession LN511711 [GenBank]
Sequencing primer gene specific primer, 44wr: 5'-GTACGTTGAGTTCAAGTTCTTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K118656-0022-389-A02C-44wr
AACGCTTCTCTTGGATTCTTAGACCGTAAGGGCTCATTGCCTCGAACACTCTTTCCGTTT
GGCTCAAGCAATCGCTAGCCGCTTCTTCACCTGGCACGAACCAGTTAGATGAGTACTGAA
TCTTAAACATGTTCCCTGCTCGGTGAGGAAACGCCGTCTCTGTCGCCGGAATCTCGCTCA
TTCTCCCGCCGTATGGGTTCCAAGCCATCGACACGTTCTCGTTCTCTGAAAGAATCTTGA
ATATCGATTCTAGACCGGGTTTTGAGATCGGTTTCTGCACGTAATCGGATTTTCTTTTAA
GGTAGATTTGCTTTTGTGGGATTCGGTTTAGCATGACCGATGTTGGTGCTGTACCCGGTG
GGATACTAAGCCAGAACAGAACTGATTCGATCCATTTCATTTCTATGCACTCTGTTTTGT
TCAGACCGAGTTCCGGGAATTTGGCGTTCATAATCTCTAGAAGCTTCTCGGAGTTTCCTA
AGTACATTCCCATGAAAGAAGCCTTGACCGTCTTGTTACTTGAGCTCAACGTTAATCTTA
TGAACAAATCGTTATCGATCTTGTCGGCGATCTCTTGCCATTAAAGGATCTATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 40-K118655-0022-389-A02C-8409
GenBank Accession LN503876 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K118655-0022-389-A02C-8409
GCCATTTACATTGAATAGATCCTTTAATGGCAAGAGATCGCCGACAAGATCGATAACGAT
TTGTTCATAAGATTAACGTTGAGCTCAAGTAACAAGACGGTCAAGGCTTCTTTCATGGGA
ATGTACTTAGGAAACTCCGAGAAGCTTCTAGAGATTATGAACGCCAAATTCCCGGAACTC
GGTCTGAACAAAACAGAGTGCATAGAAATGAAATGGATCGAATCAGTTCTGTTCTGGCTT
AGTATCCCACCGGGTACAGCACCAACATCGGTCATGCTAAACCGAATCCCACAAAAGCAA
ATCTACCTTAAAAGAAAATCCGATTACGTGCAGAAACCGATCTCAAAACCCGGTCTAGAA
TCGATATTCAAGATTCTTTCAGAGAACGAGAACGTGTCGATGGCTTGGAACCCATACGGC
GGGAGAATGAGCGAGATTCCGGCGACAGAGACGGCGTTTCCTCACCGAGCAGGGAACATG
TTTAAGATTCAGTACTCATCTAACTGGTTCGTGCCAGGTGAAGAAGCGGCTAGCGATTGC
TTGAGCCAAACGGAAAGAGTGTTCGAGGCAATGAGCCCTTACGTGTCTAAGAATCCAAGA
GAAGCGTTTTTGAACTACAGAGATATTGACATTGGGAAGAACTTGA

Last Updated on 06.12.2018 16:11