Sequences from confirmation amplicon


Confirmation sequences for line 389G01 and hit At3g07330 are shown.
Line ID 389G01
Locus At3g07330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2338657
Primers used for PCR JV67 - 8474, show primer details
   
Sequence Name 05-K101324-0022-389-G01C-8409
GenBank Accession LN488285 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K101324-0022-389-G01C-8409
CGGACTGAGCCTTTACATTGAATATATCCTGTAAATGGATCAGCCATGTATAAACCGAAG
GCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCT
TATCAGTGAAATCAGTTTAAACTATCGTTGGCAATGCGGCGATTGGCGGTGCGAACAAGG
TAAAGGAAAGAGTTACCGATCCAGAGGAAGGAAGATGCGAGTTGCTTGAACTTGAGGAGG
TAGAGACGAGAGAGCTGACGAACGGTACGAGTCCGGATCCGATCCTTATCAGGGTCAACG
GTGGCCGGAGTACGGATTTCAACGGTGAGGAATGCTTCATCGTCGCCGGCGTAAAGAACG
TCGTGATTGTTCCTATCTCGTTGCTTGTTCCACCATTGTTGAAACTCCTCGTTCTGGGAG
CGAGACATTCAACGAAGAACAAAAGAAGGATCTCTCTCGAAGCTGATGAAATCAGGAATC
GTCGATCTGATTAGATCTGAGAATTTGGGAGACAAGCGCAGAGAGAACACGATTGGGCTA
AACTTACTAACAATAGATGACGTCGTCTTTCGTCCCAGTGTCTTCCTCCCATTTCTTTTC
TTTTTTCTCTTTTTTAAAATATACATCTATAAAATTAATATTTATAAGTGATTTAATTTT
TTTTGTCCTCTTGATTTAATTAATATCAATTAGGGGATATAATTAGAGTTAAATGGTTGT
CTAGACGAATCATGTGTTAGATGCTTAAGAGGGTCTTCCAA
   
Sequence Name 05-K101341-0022-389-G01C-JV67
GenBank Accession LN488286 [GenBank]
Sequencing primer gene specific primer, JV67: 5'-TGGAAGACCCTCTTAAGCATCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K101341-0022-389-G01C-JV67
CATTTACTCTAATTTATCCCCTAATTGATATTAATTAAATCAAGAGGACAAAAAAAATTA
AATCACTTATAAATATTAATTTTATAGATGTATATTTTAAAAAAGAGAAAAAAGAAAAGA
AATGGGAGGAAGACACTGGGACGAAAGACGACGTCATCTATTGTTAGTAAGTTTAGCCCA
ATCGTGTTCTCTCTGCGCTTGTCTCCCAAATTCTCAGATCTAATCAGATCGACGATTCCT
GATTTCATCAGCTTCGAGAGAGATCCTTCTTTTGTTCTTCGTTGAATGTCTCGCTCCCAG
AACGAGGAGTTTCAACAATGGTGGAACAAGCAACGAGATAGGAACAATCACGACGTTCTT
TACGCCGGCGACGATGAAGCATTCCTCACCGTTGAAATCCGTACTCCGGCCACCGTTGAC
CCTGATAAGGATCGGATCCGGACTCGTACCGTTCGTCAGCTCTCTCGTCTCTACCTCCTC
AAGTTCAAGCAACTCGCATCTTCCTTCCTCTGGATCGGTAACTCTTTCCTTTACCTTGTT
CGCACCGCCNATCGCCGCANTGCCNACGATNGTTTAAACTGATTTCACTGATNAGGAAGT
TCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCCTCGGT
TTATACATGGCTGATCCATTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTTCATTCAAAATGT

Last Updated on 06.12.2018 16:11