Sequences from confirmation amplicon


Confirmation sequences for line 390B07 and hit At1g02400 are shown.
Line ID 390B07
Locus At1g02400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:488441
Primers used for PCR MO14 - 8474, show primer details
   
Sequence Name 19-K027064-022-390-B07C-8409
GenBank Accession LN494612 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K027064-022-390-B07C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATCAAAATTGGAGACGTCAACG
AGTAAAGATTTGACTTGTTATTCCTCTTCGTCATTTTTAAAATGACAAGTTGTAAATAAA
AAGCAGGAATCATGGGGTCCTCACTTTCCGAACATTCCTCTGTCTCATGTCTTTTCTTTT
TCTGTTATTGGTATTTATCGTAATAATAATAATAATTTGAAAATCTTTCAGAAAAAATAA
GAACAACAACCCGTCTCTCTGTCAATTGTTGTTGAGTGTGTTAATAAATTCCACGTGTTG
TTTCTCTTTTATTAGCTCGGCGACGAATGATTACATACGCACGGTTAGAGATTTGGCATG
TGAGATCATTGATCTGACGATCGAGAATTTATGGGGACAGAAGTCTAG
   
Sequence Name 20-K027064-022-390-B07C-MO14
GenBank Accession LN495064 [GenBank]
Sequencing primer gene specific primer, MO14: 5'-ACTCACTTCGCTAGACTTCTGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K027064-022-390-B07C-MO14
CCATAAATTCTCGATCGTCAGATCAATGATCTCACATGCCAAATCTCTAACCGTGCGTAT
GTAATCATTCGTCGCCGAGCTAATAAAAGAGAAACAACACGTGGAATTTATTAACACACT
CAACAACAATTGACAGAGAGACGGGTTGTTGTTCTTATTTTTTCTGAAAGATTTTCAAAT
TATTATTATTATTACGATAAATACCAATAACAGAAAAAGAAAAGACATGAGACAGAGGAA
TGTTCGGAAAGTGAGGACCCCATGATTCCTGCTTTTTATTTACAACTTGTCATTTTAAAA
ATGACGAAGAGGAATAACAAGTCAAATCTTTACTCGTTGACGTCTCCAATTTTGATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTCCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG

Last Updated on 06.12.2018 16:11