Sequences from confirmation amplicon


Confirmation sequences for line 390H01 and hit At4g24630 are shown.
Line ID 390H01
Locus At4g24630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12715271
Primers used for PCR 11kn - 8474, show primer details
   
Sequence Name 60-K113845-0022-390-H01C-11kn
GenBank Accession LN510314 [GenBank]
Sequencing primer gene specific primer, 11kn: 5'-ATTAGGCAGTAAGCAAACAGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K113845-0022-390-H01C-11kn
TTACACAATGAGATATATAGGACTATCAGATTGGGTATCGGAGAAGACTTACATAAATAG
TGAAAAGAATTGCTACCACCATGATAGCATATCCCGCATTGTAGGGAGAGAACTCATGGC
GAAGGTGCCTTGCTACAAATACACAGAACAAAACAACCGGAACTATGATCAATAGCAAGG
TAAGAGGCAGTGACCTAGCATCTGGTCCAAATATCAATCTCCCACCCAGGATGAATTTCT
GCAAAAGAGAGAGGGACCATTTATTGGTATCACCATACTGAATTTAAAACATGTGAAGAG
TCAAGCATTGAAGATACTAATTGTAAATGGCTTCATGTCCGGGATACCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGC
   
Sequence Name 33-K113844-0022-390-H01C-8409
GenBank Accession LN501076 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K113844-0022-390-H01C-8409
TTACATTAGTATCTTCAATGCTTGACTCTTCACATGTTTTAAATTCAGTATGGTGATACC
AATAAATGGTCCCTCTCTCTTTTGCAGAAATTCATCCTGGGTGGGAGATTGATATTTGGA
CCAGATGCTAGGTCACTGCCTCTTACCTTGCTATTGATCATAGTTCCGGTTGTTTTGTTC
TGTGTATTTGTAGCAAGGCACCTTCGCCATGAGTTCTCTCCCTACAATGCGGGATATGCT
ATCATGGTGGTAGCAATTCTTTTCACTATTTATGTAAGTCTTCTCCGATACCCAATCTGA
TAGTCCTATATATCTCATTGTTGTAAATATTTGATTTCTGATTTTATGTTTGTTTCCTGT
TTGCTTACTAGCCTAA

Last Updated on 06.12.2018 16:11