Sequences from confirmation amplicon


Confirmation sequences for line 390H07 and hit At1g79640 are shown.
Line ID 390H07
Locus At1g79640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29970363
Primers used for PCR 18dl - 8474, show primer details
   
Sequence Name 22-K109936-0022-390-H07C-18dl
GenBank Accession LN496155 [GenBank]
Sequencing primer gene specific primer, 18dl: 5'-TTTTGGAGATGAAATCCAGGTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K109936-0022-390-H07C-18dl
AGAAGGAGCGTGACCATGAGCAAGCTCTAGCCCAGTTATACCAAACGACCAAATATCAGC
CCTGAAGGAGATGCTCCAAATAAGATATACTATATATTCATCCAATAAGCGAATAAATAG
AATGATATAACTTTGTATAAGGCTAAACTAACTTGAAGTCATAACCATGTAGCTGCTCCA
TGACTTCAGGTGCCATCCTGTGACAAGAAGAAATAGTAGTAGGTCATGCACATGAGAACT
AACCTCAAACACTGTTAATGTCCTCCACACAAAAAAAATATAAGAAGGTATTGGGGGCAG
TAGTTAGCAACTAGCGTGGGTACTTACCAGCAAGGTGTTCCAACAAATGTGTTCCTTGTC
CGTTGCCTATCACCTGAATCAAAGAGACAGGCAGATACACCAATGGCTTCATGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 06-K109935-0022-390-H07C-8409
GenBank Accession LN488858 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K109935-0022-390-H07C-8409
TCGGACTNAGCCATTTACATGAAGCCATTGGTGTATCTGCCTGTCTCTTTGATTCAGGTG
ATAGGCAACGGACAAGGAACACATTTGTTGGAACACCTTGCTGGTAAGTACCCACGCTAG
TTGCTAACTACTGCCCCCAATACCTTCTTATATTTTTTTTGTGTGGAGGACATTAACAGT
GTTTGAGGTTAGTTCTCATGTGCATGACCTACTACTATTTCTTCTTGTCACAGGATGGCA
CCTGAAGTCATGGAGCAGCTACATGGTTATGACTTCAAGTTAGTTTAGCCTTATACAAAG
TTATATCATTCTATTTATTCGCTTATTGGATGAATATATAGTATATCTTATTTGGAGCAT
CTCCTTCAGGGCTGATATTTGGTCGTTTGGTATAACTGGGCTAGAGCTTGCTCATGGTCA
CGCTCCTTTCTCTAAATATCCACCAATGAAGGTTCATAGCCTGTGATACCTGGATTTCAT
CTCCAAA

Last Updated on 06.12.2018 16:11