Sequences from confirmation amplicon


Confirmation sequences for line 390H08 and hit At5g55400 are shown.
Line ID 390H08
Locus At5g55400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22457095
Primers used for PCR 11kt - 8474, show primer details
   
Sequence Name 41-K113817-0022-390-H08C-11kt
GenBank Accession LN504153 [GenBank]
Sequencing primer gene specific primer, 11kt: 5'-ACTGAGCCTGGATAGACCTTATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K113817-0022-390-H08C-11kt
TATTCAGTATTGAAAACTTATAATACGTTTCAGGACCAAAAGATTATAAATTATTTTGCA
TACCAAATGCTTACCCGTTTCTAACATCTTCAAACACATTATTGACGTAACTCTCAATCC
CGAGGCTGTTAATCCATAGCCGGTAACATCTTTCGTCTCTGCAAGTCTGTAAATCCTCAG
TCATCATCTCCGCAAAGGAGAATCTACCATCAGTGCTAAGGCCATTCCTGCAGCAGGTAC
AGAGAAGCTAGTGTGTAAGAAACCAGTACATAAGAAAATATTCTATCTACATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 15-K113816-0022-390-H08C-8409
GenBank Accession LN493055 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K113816-0022-390-H08C-8409
TGAAATTTTCTTATGTACTGGTTTCTTACCACTAGCTTCTCTGTACCTGCTGCAGGAATG
GCCTTAGCACTGATGGTAGATTCTCCTTTGCGGAGATGATGACTGAGGATTTACAGACTT
GCAGAGACGAAAGATGTTACCGGCTATGGATTAACAGCCTCGGGATTGAGAGTTACGTCA
ATAATGTGTTTGAAGATGTTAGAAACGGGTAAGCATTTGGTATGCAAAATAATTTATAAT
CTTTTGGTCCTGAAACGTATTATAAGTTTTTCAATACTGAATATTTCTGATGCAGATGGA
TTCTTCTGGAAGTTGTTGATAAGGTCTATCCAGGCTCAG

Last Updated on 06.12.2018 16:11