Sequences from confirmation amplicon


Confirmation sequences for line 391D01 and hit At4g09300 are shown.
Line ID 391D01
Locus At4g09300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5899464
Primers used for PCR BW91 - 8474, show primer details
   
Sequence Name 30-K106020-0022-391-D01C-BW91
GenBank Accession LN499725 [GenBank]
Sequencing primer gene specific primer, BW91: 5'-ATTTCGGGATTCGATAGCTTTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K106020-0022-391-D01C-BW91
GCAGATCTTCCTCTGCTGTTTCTGGTTAAGGAAAAAAGAACTGAAAGCAGAAAGTTATTT
CACAAGTTTAAACTTGAATCATCACCATCAGAAGAGAACGAATCTGAGAAATGTCTTCCA
CACTTTTAAAGCAGCATCATCATCAGAAGAGAAAGAATCTAACAAAAGTTCTTTTACAAG
TTTAAACAAGAATCATCATCATCAGAAGAGAACGAATCTGAGAAAGTTCTTACACAAATC
ACAAGTTTAAACATGAATCATCATCCGAATCAGAAGAGAACGAATCTAATAAATTCCTTT
CACTAGTTTAAACACGAATCATCATCAGAGAAATAATCTGAGAAATTTCATTCACACTCT
CACATCATCATATTAAGAGAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 30-K106021-0022-391-D01C-8409
GenBank Accession LN499726 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K106021-0022-391-D01C-8409
GCCATTTTTCTCTTATATGATGATGTGAGAGTGTGAATGAAATTTCTCAGATTATTTCTC
TGATGATGATTCGTGTTTAAACTAGTGAAAGGAATTTATTAGATTCGTTCTCTTCTGATT
CGGATGATGATTCATGTTTAAACTTGTGATTTGTGTAAGAACTTTCTCAGATTCGTTCTC
TTCTGATGATGATGATTCTTGTTTAAACTTGTAAAAGAACTTTTGTTAGATTCTTTCTCT
TCTGATGATGATGCTGCTTTAAAAGTGTGGAAGACATTTCTCAGATTCGTTCTCTTCTGA
TGGTGATGATTCAAGTTTAAACTTGTGAAATAACTTTCTGCTTTCAGTTCTTTTTTCCTT
TAACCAGAAACAGCAGAGGAAGATCTTGCTAGCATGAATGAGCGATTGGAGGTGATAAAA
GCTATCGAATCCCGAAA

Last Updated on 06.12.2018 16:11