Sequences from confirmation amplicon


Confirmation sequences for line 391E04 and hit At3g06190 are shown.
Line ID 391E04
Locus At3g06190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1874732
Primers used for PCR 13pk - 8474, show primer details
   
Sequence Name 57-K115187-0022-391-E04C-13pk
GenBank Accession LN509503 [GenBank]
Sequencing primer gene specific primer, 13pk: 5'-CCGCATGCAGTAAATACATAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K115187-0022-391-E04C-13pk
AATTTTCTGCTTTCAGCTTTGCTTTTTTTTGTTAGATTGGTCTTTAGTCGATGTGCGAGT
TTTAGATCATGGCTCCTGTAAATTTAAGGTTGATACAAATTCCCTTCCTTTTGGTTTATT
GTACTTTTTTGTTGTTGTTGCAGATGTTGCTTCATTTTATCTACTGGGATGAAATGCCTG
ATATGCAAGACTTAATCGGCACAGACTTGAAATGGGCATCAACTCTTGTGGCTCAGCATC
TGCTTGCAGCTGCAGACCGTTATGCTCTTGAGCGGCTTAGAACAATATGCGAGTCAAAAC
TCTGTGAAGGAATCAGCATAAATACGGTAGCAACCACCTTGGCTCTTGCAGAGCAGCATC
ATTGTTTCCAGCTGAAAGCCGCCTGCCTCAAATTTATAGCTTTGCCAGAAAACCTGAAAG
GTACAGAAGATCACTCCTTGCTTGTTTGAATCGCTTTGATCATGTCTGAAATCTGAAGGC
CGCCCTTGTAACAAAAAGTTGTCTGTAATTTCTTTAGCTGTGATGGAAACGGATGGGTTT
GATTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCG
   
Sequence Name 21-K115186-0022-391-E04C-8409
GenBank Accession LN495784 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K115186-0022-391-E04C-8409
TTTACATTGAAAATCAAACCCATCCGTTTCCATCACAGCTAAAGAAATTACAGACAACTT
TTTGTTACAAGGGCGGCCTTCAGATTTCAGACATGATCAAAGCGATTCAAACAAGCAAGG
AGTGATCTTCTGTACCTTTCAGGTTTTCTGGCAAAGCTATAAATTTGAGGCAGGCGGCTT
TCAGCTGGAAACAATGATGCTGCTCTGCAAGAGCCAAGGTGGTTGCTACCGTATTTATGC
TGATTCCTTCACAGAGTTTTGACTCGCATATTGTTCTAAGCCGCTCAAGAGCATAACGGT
CTGCAGCTGCAAGCAGATGCTGAGCCACAAGAGTTGATGCCCATTTCAAGTCTGTGCCGA
TTAAGTCTTGCATATCAGGCATTTCATCCCAGTAGATAAAATGAAGCAACATCTGCAACA
ACAACAAAAAAGTACAATAAACCAAAAGGAAGGGAATTTGTATCAACCTTAAATTTACAG
GAGCCATGATCTAAAACTCGCACATCGACTAAAGACCAATCTTAACAAAAAAAAGCAAAG
CTGAAAAGCAGAAAATTTGACATGAACATGATGATCTATAACATAATTTCTCTATG

Last Updated on 06.12.2018 16:11