Sequences from confirmation amplicon


Confirmation sequences for line 391E05 and hit At3g14080 are shown.
Line ID 391E05
Locus At3g14080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4668422
Primers used for PCR MI23 - 8474, show primer details
   
Sequence Name 21-K024724-022-391-E05C-8409
GenBank Accession LN495504 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K024724-022-391-E05C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATTCGGAACACTCCGTTCCTTTGATCA
ATTCGGTATTCACTTTTCTTTCCATGTTTCTAGATTTACGATATTGTTGATGAGCGGGAG
AGATTTGACTTTTTTTTGTTCGTTGTAAATACACAGCCAATGCGGTTCTAGAAGGTGCGT
GTGAGAGGGTAATTGTTGGTGAACAATACTGTGACATTCCTTTAGGCCTCTATGTAATCC
GTGGAGAGAATGTTGTTTTGATTGGTGAGCTGGTAAGACTTCTCCTTGTTTGTCTTTTCT
TGCTGTGTCACCTCGTGTTTTCCAATTCACTGAGTTTGACCTTAAACTATGTATTAGGAC
ACGGAGAGAGAAGAGCTTCCTCCACATATGATTCGCGTCTCAGAGGCAGAGATTAAAAGG
GTAAGACATCGCATAACATGTTCATTTTGATTAGCCAAATTTGATTCATTTGTGATGCTG
TGTGCGTGCTGATGATTTGGTTTTCGGGAATCTGTCTGTTGATGCAGGCACAAAAGGTGG
AGAGGGAAGCGAGT
   
Sequence Name 22-K024724-022-391-E05C-MI23
GenBank Accession LN495951 [GenBank]
Sequencing primer gene specific primer, MI23: 5'-CCAATAATGAATGGGGATACAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K024724-022-391-E05C-MI23
TTTAATCAAAGTCAAGAAACTCCATTCTCTTCCTCATTGTTCCTCTCAGCTCACTCGCTT
CCCTCTCCACCTTTTGTGCCTGCATCAACAGACAGATTCCCGAAAACCAAATCATCAGCA
CGCACACAGCATCACAAATGAATCAAATTTGGCTAATCAAAATGAACATGTTATGCGATG
TCTTACCCTTTTAATCTCTGCCTCTGAGACGCGAATCATATGTGGAGGAAGCTCTTCTCT
CTCCGTGTCCTAATACATAGTTTAAGGTCAAACTCAGTGAATTGGAAAACACGAGGTGAC
ACAGCAAGAAAAGACAAACAAGGAGAAGTCTTACCAGCTCACCAATCAAAACAACATTCT
CTCCACGGATTACATAGAGGCCTAAAGGAATGTCACAGTATTGTTCACCAACAATTACCC
TCTCACACGCACCTTCTAGAACCGCATTGGCTGTGTATTTACAACGAACAAAAAAAAGTC
AAATCTCTCCCGCTCATCAACAATATCGTAAATCTAGAAACATGGAAAGAAAAGTGAATA
CCGAATTGATCAAAGGAACGGAGTGTTCCGAATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATG

Last Updated on 06.12.2018 16:11