Sequences from confirmation amplicon


Confirmation sequences for line 392D02 and hit At5g11250 are shown.
Line ID 392D02
Locus At5g11250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3588560
Primers used for PCR BG70 - 8474, show primer details
   
Sequence Name 10-K104021-0022-392-D02C-BG70
GenBank Accession LN490626 [GenBank]
Sequencing primer gene specific primer, BG70: 5'-TATAATGTGCCCCAATGTTGTAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K104021-0022-392-D02C-BG70
TACCATTTGTCTCTATCATGAGCGCCATAGTCACCAATACGAACTAGCAAGATGCAAGCC
TTATATCTCATAGATGTAGGAAGAGGCCTCTCATTCAACTTAATTGTCAATGAACCCCCA
CTCGCTCGGTGAGTGAAGTATGCAGGCACCTCTCTACCAGGTAAGACTGCTTGTTTAGTC
GGTGTCTGGATGATTAGGTCTCTTGCTTCTTGATTAAGTTTAAAGCACTTACCAAAGAAG
AGAGTAATCTCTGGATTGTGAAAGGAGCAATCTAGTCTCTCCAAGGACTCACAATCTTCA
GCATCAATCCATTTTAAAGAATCTGGAATCTGTGGGAGTGATACCACCTTTCTGTACCCC
TTGAGTATGAGTGTCTGAAGACGAGATATTCTCTTGATCCATGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 26-K104022-0022-392-D02C-8409
GenBank Accession LN497894 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104022-0022-392-D02C-8409
GCCATTTACTGGATCAAGAGAATATCTCGTCTTCAGACACTCATACTCAAGGGGTACAGA
AAGGTGGTATCACTCCCACAGATTCCAGATTCTTTAAAATGGATTGATGCTGAAGATTGT
GAGTCCTTGGAGAGACTAGATTGCTCCTTTCACAATCCAGAGATTACTCTCTTCTTTGGT
AAGTGCTTTAAACTTAATCAAGAAGCAAGAGACCTAATCATCCAGACACCGACTAAACAA
GCAGTCTTACCTGGTAGAGAGGTGCCTGCATACTTCACTCACCGAGCGAGTGGGGGTTCA
TTGACAATTAAGTTGAATGAGAGGCCTCTTCCTACATCTATGAGATATAAGGCTTGCATC
TTGCTAGTTCGTATTGGTGACTATGGCGCTCATGATAGAGACAAATGGTTACATGTTGCT
TTCGGTGTTAGGTCTACAACATTGGGGCACATTAT

Last Updated on 06.12.2018 16:11