Sequences from confirmation amplicon


Confirmation sequences for line 393B05 and hit At1g80320 are shown.
Line ID 393B05
Locus At1g80320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30197734
Primers used for PCR FQ85 - 8474, show primer details
   
Sequence Name 61-K033877-022-393-B05C-FQ85
GenBank Accession LN510444 [GenBank]
Sequencing primer gene specific primer, FQ85: 5'-ATCCCTTCTGGTAAAAACACACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K033877-022-393-B05C-FQ85
CTATGTCGTTCTTGTCACATCTCAGATGATTTGCAGTCAAACCACACATATGTATGCAAT
GACAATGGCGGAGCTAGACCAGACGGTGATGAGGATGTTGTATGAAAGCTATGGAATGGA
TGAGAAGAAGCATTCTGTGAGTCACTCCGAGTCAACTCGATACCTGTTGAGGATGCTGAG
TTACAGGAGACAGCAAAACGGTGAAGCAAACACTGGTTTCGTGTCACACACTGATAAGAG
CTTCATGAGTATCCTCCACCAGAACCACGTCGGAGGTCTCCAGTTGAAAACCATGACCGG
CCAATGGGTCGGGTTTAACCCGTCCCCAACAAGATTCGTCGTTCTCTCCGGCATGGGTCT
CACGGTGAGCTCTAAACCAAATCCGATTAGTCGAAACACCTAGACATAGCAATAAATGTG
AAATCTTTTTGTTGGTGATGAAGGCGTGGAGTAACGATAGGATAAAGGCATGTTACCACA
AGGTAGTGATGAGTGCAGATGAGATTCGATACTCTTTAGGGTTTTTCTCCTTCCACAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 05-K033877-022-393-B05C-8409
GenBank Accession LN488251 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K033877-022-393-B05C-8409
GAAGCCCATTTGTGGAAGGAGAAAAACCCTAAAGAGTATCGAATCTCATCTGCACTCATC
ACTACCTTGTGGTAACATGCCTTTATCCTATCGTTACTCCACGCCTTCATCACCAACAAA
AAGATTTCACATTTATTGCTATGTCTAGGTGTTTCGACTAATCGGATTTGGTTTAGAGCT
CACCGTGAGACCCATGCCGGAGAGAACGACGAATCTTGTTGGGGACGGGTTAAACCCGAC
CCATTGGCCGGTCATGGTTTTCAACTGGAGACCTCCGACGTGGTTCTGGTGGAGGATACT
CATGAAGCTCTTATCAGTGTGTGACACGAAACCAGTGTTTGCTTCACCGTTTTGCTGTCT
CCTGTAACTCAGCATCCTCAACAGGTATCGAGTTGACTCGGAGTGACTCACAGAATGCTT
CTTCTCATCCATTCCATAGCTTTCATACAACATCCTCATCACCGTCTGGTCTAGCTCCGC
CATTGTCATTGCATACATATGTGTGGTTTGACTGCAAATCATCTGAGATGTGACAAGAAC
GACATAGATGAAATGAAAGAAAGTCTTTATGTGTGTGTTTTTCCAGAAAGGG

Last Updated on 06.12.2018 16:11