Sequences from confirmation amplicon


Confirmation sequences for line 393B05 and hit At5g27380 are shown.
Line ID 393B05
Locus At5g27380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9669032
Primers used for PCR RE42 - 8474, show primer details
   
Sequence Name 19-K026237-022-393-B05C-8409
GenBank Accession LN494606 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K026237-022-393-B05C-8409
ACCATTCCTGCAAATTGAAACAAATAAACCAATGATATGATTCAGATTTTCCCAAAGAGT
AAAGCCACAATATTGGAAACTTGACGGCAACTTACTGATTCAGACGGATGATCATTAGGA
GTATAGCCAGATCTGAAATAAACCACTGCGACTGCTTGGCCGCCACTGCAACAATGAAAA
CTCTTAACATAGCTTTCAAGATGAAGGTGTAAGTTCTCAAAAAACCACAATGCAAACAAA
TGCTTACACAATAAGGGTTTCATCCTCTTGTACACTCCCTTCTTTTTCGACTTCTGCTAG
AGTCTTCCTGATGACAACTATATTATGCGTGTTCTGAAGCTAAGGAAGAACAACACCATT
TTTTGTGGTACAAAATATAGCATAAGAAGTCAAGATCGACCCCTGGAAACCAAAGAAACA
AGACAAAAGAGGATATTTTTCTCTCAATATACTGCTCAGCAAATGTTGATCGTACATGTT
GCGTTCTTCTGGCTGTACAA
   
Sequence Name 20-K026237-022-393-B05C-RE42
GenBank Accession LN495060 [GenBank]
Sequencing primer gene specific primer, RE42: 5'-AGTCATGGTAATTGTACAGCCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K026237-022-393-B05C-RE42
CGCACATGTACGATCAACATTTGCTGAGCAGTATATTGAGAGAAAAATATCCTCTTTTGT
CTTGTTTCTTTGGTTTCCAGGGGTCGATCTTGACTTCTTATGCTATATTTTGTACCACAA
AAAATGGTGTTGTTCTTCCTTAGCTTCAGAACACGCATAATATAGTTGTCATCAGGAAGA
CTCTAGCAGAAGTCGAAAAAGAAGGGAGTGTACAAGAGGATGAAACCCTTATTGTGTAAG
CATTTGTTTGCATTGTGGTTTTTTGAGAACTTACACCTTCATCTTGAAAGCTATGTTAAG
AGTTTTCATTGTTGCAGTGGCGGCCAAGCAGTCGCAGTGGTTTATTTCAGATCTGGCTAT
ACTCCTAATGATCATCCGTCTGAATCAGTAAGTTGCCGTCAAGTTTCCAATATTGTGGCT
TTACTCTTTGGGAAAATCTGAATCATATCATTGGTTTATTTGTTTCAATTTGCAGGAATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11