Sequences from confirmation amplicon


Confirmation sequences for line 393H02 and hit At1g26390 are shown.
Line ID 393H02
Locus At1g26390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9131372
Primers used for PCR BW98 - 8474, show primer details
   
Sequence Name 09-K106136-0022-393-H02C-8409
GenBank Accession LN490186 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K106136-0022-393-H02C-8409
GAGCCTTTACAATTGAATACCGTAGTCATGACCGCCGCTTCGTATACGGAGCTGGATACC
ATTGGACTTTGCGCAGACCACCGTGGCCTGAACATGAGATACATGTTTTGCGGCCACGAT
GGCTAGTAGCTTTCTATAATTAGGGGTCGAGAACCTTTTGTTTTTAGTGTATGACACGTA
AGAAGACAAGAAGGTGGTGGTGTTATCGGCGAAGGAGATGGCATCGGTGATTGGATTCTC
CGGACTAGTACGATAGCGAAGGCACCCGATGAAGTCTCCAAATTTTGGTTTTGTTACTGC
TGCTTCCAAAACCGAAACCAGAAGTACAAG
   
Sequence Name 09-K106135-0022-393-H02C-BW98
GenBank Accession LN490185 [GenBank]
Sequencing primer gene specific primer, BW98: 5'-TCTTGTACTTCTGGTTTCGGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K106135-0022-393-H02C-BW98
ATTTGGAGACTTATCGGGTGCCTTCGCTATCGTACTAGTCCGGAGAATCCAATCACCGAT
GCCATCTCCTTCGCCGATAACACCACCACCTTCTTGTCTTCTTACGTGTCATACACTAAA
AACAAAAGGTTCTCGACCCCTAATTATAGAAAGCTACTAGCCATCGTGGCCGCAAAACAT
GTATCTCATGTTCAGGCCACGGTGGTCTGCGCAAAGTCCAATGGTATCCAGCTCCGTATA
CGAAGCGGCGGTCATGACTACGGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCG
Primers used for PCR BW99 - 8474, show primer details
   
Sequence Name 10-K106136-0022-393-H02C-OriL
GenBank Accession LN490664 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K106136-0022-393-H02C-OriL
GCCATTTACAATTTGAGGTCTATTACTGTTGACGTGTCAAGCAAGAAAGCCTGGATTCAA
GCTGGTGCGACCTTGGGAGAGCTCTACACAAACGTCAATGACGTAAGCCAAACACTAGCT
TTCCCGGCTGGCGTTTGCGCTACCGTAGGAGCTGGTGGGCACATAAGTGGTGGAGGGTAT
GGAAATCTAATGAGAAAATACGGAATCACTGTGGATCATGTCATTGATGCTCAAATAATT
GATGTCAACGGCAAGCTCTTGAATCGAGCTACCATGGGAGAAGATCTCTTTTGGGCAATC
CGTGGCGGTGGCGGTGGGAGTTTTGGAGTTATCCTCTNTTGGAAA
   
Sequence Name 10-K106135-0022-393-H02C-BW99
GenBank Accession LN490663 [GenBank]
Sequencing primer gene specific primer, BW99: 5'-TTTTCCAAGAGAGGATAACTCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K106135-0022-393-H02C-BW99
GCCCGGATTGCAAAGAGATCTTCTCCCATGGTAGCTCGATTCAGAGCTTGCCGTTGACAT
CAATTATTTGAGCATCAATGACATGATCCACAGTGATTCCGTATTTTCTCATTAGATTTC
CATACCCTCCACCACTTATGTGCCCACCAGCTCCTACGGTAGCGCAAACGCCAGCCGGGA
AAGCTAGTGTTTGGCTTACGTCATTGACGTTTGTGTAGAGCTCTCCCAAGGTCGCACCAG
CTTGAATCCAGGCTTTCTTGCTTGACACGTCAACAGTAATAGACCTCAAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATT

Last Updated on 06.12.2018 16:11