Sequences from confirmation amplicon


Confirmation sequences for line 394A05 and hit At1g52900 are shown.
Line ID 394A05
Locus At1g52900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19702231
Primers used for PCR 70yt - 8474, show primer details
   
Sequence Name 47-K122663-0022-394-A05C-70yt
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 70yt: 5'-CACTTCTAAAGTCGCTGTAACGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K122663-0022-394-A05C-70yt
CGAGCTTGCTCTTATAATGGAGTCCAGAAAAAGGGTCATACCGATATTCTGCGACATCAA
ACCTTCACAACTCGATGTTATGATCGAGAGGGTGACATGTTCTGATGATGAAATCCAAAG
GTTTAGATGGGCCCTTCAAGAAGCTAAAGATATCGTTGGACTCACGTTTGATTCCTACAA
AGGGTAATTTATTTTTTCTATCAAATCTTTTTATTAACTATCTCTGTTTTATTATTATTG
TACTTTTGTTTATTCAGTTTCTGATTTAACTCAACATTCATTTATTTAGGAATTTATCGG
AGGTTGTTACAGTTGCATCGGATGTTATCGTCGAGAGATTGGTCGAGTTAGAGGATCAAT
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 07-K122662-0022-394-A05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K122662-0022-394-A05C-8409
TGATCCTCTAACTCGACCAATCTCTCGACGATAACATCCGATGCAACTGTAACAACCTCC
GATAAATTCCTAAATAAATGAATGTTGAGTTAAATCAGAAACTGAATAAACAAAAGTACA
ATAATAATAAAACAGAGATAGTTAATAAAAAGATTTGATAGAAAAAATAAATTACCCTTT
GTAGGAATCAAACGTGAGTCCAACGATATCTTTAGCTTCTTGAAGGGCCCATCTAAACCT
TTGGATTTCATCATCAGAACATGTCACCCTCTCGATCATAACATCGAGTTGTGAAGGTTT
GATGTCGCAGAATATCGGTATGACCCTTTTTCTGGACTCCATTATAAGAGCAAGCTCGTG
CAGACAGAAATATGAATCGCAGTAGTTAGGAGAAAAAACCGTTACAGCGACTTTTAGAAG
TG

Last Updated on 06.12.2018 16:11