Sequences from confirmation amplicon


Confirmation sequences for line 394C01 and hit At2g37975 are shown.
Line ID 394C01
Locus At2g37975 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15892100
Primers used for PCR KP57 - 8474, show primer details
   
Sequence Name 51-K034067-022-394-C01C-KP57
GenBank Accession LN507415 [GenBank]
Sequencing primer gene specific primer, KP57: 5'-GGTTTGATGGTTATGAGTGGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K034067-022-394-C01C-KP57
GTAACACTGGGAAAACAATTTTATCTTTCAAGGCACACAATAATGAAAACTAGGACCTGC
CCTGATCACTGTAAAGCTTTATAGTAGTATTGCAATCGGTTCTTGTTTATTAATGTGTTA
AATTCAAGATGTTGCCTTACTACCTTCTTTGCTTTCTTCTGAAAACTTTTAAATACAAAG
TTAAGCTAAAAGTTCTCTATCTTTCAAAACACCAATTATTCAAAAGCTTGATCACTTGCA
TATAGTACCACAAATGTTCCATGTTTACTTAACACTGTATGTTACCAAGAGTATATGTTA
CCACTCTTGCCGCAAAAGTCTTGATTGTTTTCTATCCTCTCCAGTTTCCACAGAAAAATC
ATGGGGTTCTGGACACTAATGGAAGGATTGCTACTATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAAATG
G
   
Sequence Name 35-K034067-022-394-C01C-8409
GenBank Accession LN501734 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K034067-022-394-C01C-8409
TTTACAATTGAATATAGTAGCAATCCTTCCATTAGTGTCCAGAACCCCATGATTTCTCTG
TGGAAACTGGAGAGGATAGAAAACAATCAAGACTTATGCGGCAAGAGTGGTAACATATAC
TCTTGGTAACATACAGTGTTAAGTAAACATGGAACATTTGTGGTACTATATGCAAGTGAT
CAAGCTTTTGAATAATTGGTGTTCTGAAAGATAGAGAACTCTTAGCTTAACTTTGTATCT
AAAAGTTCTCAGAAGAAAGCAAAGAAGGTAGTAAGGCAACATCTTGAATCTAACACATTA
ATAAACAAGAACCGATTGCAATACTACTATAAAGCTTTACAGTGATCAGGGCAGGTCCTA
GTTTTCATTATTGTGTGCCTTGAAAGATAAAATTGTTTTCCAGTGTTACACTGTTGACAT
GAAATTGCAAAAAAAAAACTCAAGACCACTCATACCAATCAAAC

Last Updated on 06.12.2018 16:11