Sequences from confirmation amplicon


Confirmation sequences for line 394F06 and hit At5g66480 are shown.
Line ID 394F06
Locus At5g66480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26546032
Primers used for PCR 21fp - 8474, show primer details
   
Sequence Name 60-K111394-0022-394-F06C-21fp
GenBank Accession LN510293 [GenBank]
Sequencing primer gene specific primer, 21fp: 5'-GTTTATCCGTTATCGACTCTCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K111394-0022-394-F06C-21fp
CCCTATTGGTGGATTTCTAATTGAAATCTTCAATGATGCAGAGTCATGCCTACATTTTCT
GCTGCCGCTTTGGGTAGATCACTAAATTCAGGGACGTCCTTATCTAGTAAATTTCCAAGC
ACACTGCAGTCAAAGCCGTCTATTTTGAATGATGAAAGTAAGCAACCTAAAGAAAAGACA
TTCACTCGTCCTCAGATGTCACCTTCTCTATATGCCACTACTAAAGAAATCCCTCATCCC
AATTCGCCTTCTTCCTACCCTCCATCTCCCTATATCATCAATCACAAAGCTCGTGGGCCT
GTTCTTTTCAATAGGGACTCTGAGGTGGATGGGCCTTCTCACCCGATAACCTCTGGTGAG
GAAAAGATTAGTGGTAATGTAGATGTGGAGGCTACTGCCTCCTTGAGCAAGTCCACATCC
CTATCATTTCCTATCACCGAGGCTATTGCGGTGGATCACACCAATGGTGTTCACACCCAA
GGCATCCATGTAAATGGTGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 44-K111393-0022-394-F06C-8409
GenBank Accession LN505223 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K111393-0022-394-F06C-8409
TTTCATTGAACCCATTTACATGGATGCCTTGGGTGTGAACCCATTGGTGTGATCCACCGC
AATAGCCTCGGTGATAGGAAATGATAGGGATGTGGACTTGCTCAAGGAGGCAGTAGCCTC
CACATCTACATTACCACTAATCTTTTCCTCACCAGAGGTTATCGGGTGAGAAGGCCCATC
CACCTCAGAGTCCCTATTGAAAAGAACAGGCCCACGAGCTTTGTGATTGATGATATAGGG
AGATGGAGGGTAGGAAGAAGGCGAATTGGGATGAGGGATTTCTTTAGTAGTGGCATATAG
AGAAGGTGACATCTGAGGACGAGTGAATGTCTTTTCTTTAGGTTGCTTACTTTCATCATT
CAAAATAGACGGCTTTGACTGCAGTGTGCTTGGAAATTTACTAGATAAGGACGTCCCTGA
ATTTAGTGATCTACCCAAAGCGGCAGCAGAAAATGTAGGCATGACTCTGCATCATTGAAG
ATTTCAATTAGAAATCCACCAATAGGGATACACAACAGCGAGAGAGCGTTGTTCAACTAA
TAAAGTAATGAACATCGAGAGAGTCGATA

Last Updated on 06.12.2018 16:11