Sequences from confirmation amplicon


Confirmation sequences for line 394G04 and hit At2g29690 are shown.
Line ID 394G04
Locus At2g29690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12693842
Primers used for PCR 34nb - 8474, show primer details
   
Sequence Name 58-K114597-0022-394-G04C-34nb
GenBank Accession LN509777 [GenBank]
Sequencing primer gene specific primer, 34nb: 5'-CAAAATGCAAACACTGAGAACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K114597-0022-394-G04C-34nb
CGAACCGGCTCCCAGACTCGAACAAGAAGCTAGGAGCATCTCTATCATCTATCTTTCCCA
AACATCGGTAAGCAAGTATCGGAGTCAAATGATCCGAGAATACACATCGGAATAGAGGAA
CTAAGTTTCCCTTTTCCGATGCTTTTTTAAATTTTGTGAACTGTTCCTCCGATAAATCTG
TGAGGAGAAGAAGAAGTTCAACTTCGATCGAAATTGAGAGGCGCTAATAATGGGGGAGAT
CAGATTTTTACCTAACGACGGAGTATATGAGCATTTGATAGAAGGAAGACGACGCGAGAA
GTGGAGGAGTTTTGATCCAGCCACAAGGTTAAGAGAAGTAGCCGGCGGGGACTTGAGCGA
TAGTGGAAACCGGAGCGAAGGGAAATGTGGTGGATGCGGCGTCCGATGGTGTGTAACGGC
TATGGCTTCGACGGTGAAAAAATCAGATTTGACGGCGGAGATTGAAACGGCCGACATCCC
CGTGAGCTCGTTGATTTTAAGTCTTTCGATACCAGTTTAAACCTTTTTAAGTGTGGACTG
GGAGAAGAATATATACACTACAGTACTACAAATACAAAGCCCATAAAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 18-K114596-0022-394-G04C-8409
GenBank Accession LN494408 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K114596-0022-394-G04C-8409
ACACTTAAAAAGGTCTAAACTGGTATCGAAAGACTTAAAATCAACGAGCTCACGGGGATG
TCGGCCGTTTCAATCTCCGCCGTCAAATCTGATTTTTTCACCGTCGAAGCCATAGCCGTT
ACACACCATCGGACGCCGCATCCACCACATTTCCCTTCGCTCCGGTTTCCACTATCGCTC
AAGTCCCCGCCGGCTACTTCTCTTAACCTTGTGGCTGGATCAAAACTCCTCCACTTCTCG
CGTCGTCTTCCTTCTATCAAATGCTCATATACTCCGTCGTTAGGTAAAAATCTGATCTCC
CCCATTATTAGCGCCTCTCAATTTCGATCGAAGTTGAACTTCTTCTTCTCCTCACAGATT
TATCGGAGGAACAGTTCACAAAATTTAAAAAAGCATCGGAAAAGGGAAACTTAGTTCCTC
TATTCCGATGTGTATTCTCGGATCATTTGACTCCGATACTTGCTTACCGATGTTTGGTGA
AAGAAGATGATAGAGATGCTCCTAGCTTCTTGTTCGAGTCTGTGGAGCCTGGTTCGCAAT
CGTCCAACATCGTAAGCCTTCCTTTTTGATGATTGTTCTC

Last Updated on 06.12.2018 16:11