Genotyping and primer details


Genotyping and primer details for 394G04 - At2g29690
Line ID 394G04
Locus At2g29690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12693842 of line 394G04 Chr2:12693842
   
Insertion allele amplicon 8474 - 34nb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 34nb: 5'-CAAAATGCAAACACTGAGAACAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12694436
   
wt amplicon (over InsPos) 69rd - 34nb
wt amplicon start Chr2:12693660
wt amplicon end Chr2:12694436
wt amplicon size 777 bp
Locus specific primer 34nb: 5'-CAAAATGCAAACACTGAGAACAAT-3'
2nd locus specific primer 69rd: 5'-AGCAAACCCCTAAGGAATAAATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11