Sequences from confirmation amplicon


Confirmation sequences for line 394H03 and hit At1g77410 are shown.
Line ID 394H03
Locus At1g77410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29090698
Primers used for PCR 22yh - 8474, show primer details
   
Sequence Name 84-K119874-0022-394-H03C-22yh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 22yh: 5'-TATTGTTTCTATGGTGCCTCAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K119874-0022-394-H03C-22yh
TCAAGGACCAATTCACCCAACAAACCTGCAATATGGACTGAGAACTGGACGAGTTTGTAT
GGTCTTCACTCAAATTCACTACATTATTGTGTACTTCCCAATCCCCAGATATAAACTACT
AACTTTCAGCTGACCCCTTTGTATAGTTATCAAACATATGGTGAGGAACCATTGATAAGG
TCAGCTGAGGATATAGCGTTTCATGTTGCTCTTTTCATCGCTAAGAATGGAAGCTTTGTA
AACTACTACATGGTACCTTTTCCAGTCTCACTAAAAAAATCTCTAATGTATAAGAGAAGC
AAGAATAATGATGATTTGACATTGTATCTTATTTTAGTATCATGGAGGAACCAACTTCGG
GAGAAATGCTTCACAGTTCGTAATCACCAGTTATTATGATCAGGCTCCACTTGATGAATA
TGGTAATATTTCAAACCATTCACAGTCAAAATCAGATGTTCATCTGAAAGTACAACATAA
ATGGTTGGTGCTGCAGGGTTACTTAGGCAACCAAAATGGGGACACCTTAAGGAATTACAT
GCTGCAGTTAAGTTGTGTGAAGAGCCTCTACTTTCAGGACTGCAAACTACAATATCATTG
GGGAAGCTACAAACTGTAAGAAAATTCTAACAGTATCGTGATATCATGAAGAATGTTGTA
ATAGTGATCTTTACGTCTTATTTAACAGGCTTTTGTGTTTGGGAAGAAGGCGAACCTATG
CGCTGCCATTCTGGTGAATCAAGACAAATGCGAGTCTACTGTACAGTTTCGTAATTCTTC
ATATCGTTTGTCTCCAAAATCTGTAAGTGTCCTGCCAGACTGCAAGAATGTAG
   
Sequence Name 60-K119873-0022-394-H03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K119873-0022-394-H03C-8409
CATTTCATTGAATATTTGTATGAGCTTACCTTGGCAGTGTTGAAGGCTACATTCTTGCAG
TCTGGCAGGACACTTACAGATTTTGGAGACAAACGATATGAAGAATTACGAAACTGTACA
GTAGACTCGCATTTGTCTTGATTCACCAGAATGGCAGCGCATAGGTTCGCCTTCTTCCCA
AACACAAAAGCCTGTTAAATAAGACGTAAAGATCACTATTACAACATTCTTCATGATATC
ACGATACTGTTAGAATTTTCTTACAGTTTGTAGCTTCCCCAATGATATTGTAGTTTGCAG
TCCTGAAAGTAGAGGCTCTTCACACAACTTAACTGCAGCATGTAATTCCTTAAGGTGTCC
CCATTTTGGTTGCCTAAGTAACCCTGCAGCACCAACCATTTATGTTGTACTTTCAGATGA
ACATCTGATTTTGACTGTGAATGGTTTGAAATATTACCATATTCATCAAGTGGAGCCTGA
TCATAATAACTGGTGATTACGAACTGTGAAGCATTTCTCCCGAAGTTGGTTCCTCCATGA
TACTAAAATAAGATACAATGTCAAATCATCATTATTCTTGCTTCTCTTATACATTAGAGA
TTTTTTTAGTGAGACTGGAAAAGGTACCATGTAGTAGTTTACAAAGCTTCCATTCTTAGC
GATGAAAAGAGCAACATGAAACGCTATATCCTCAGCTGACCTTATCAATGGTTCCTCACC
ATATGTTTGATAACTATACAAAGGGGTCAGCTGAAAGTTAGTAGTTTATATCTGGGGGAT
TNGGAAGTACACAATAATGTAGTGAATTTGAGTGAAGACCATACAAACTCGTCCAGTTCT
CAGTCCATATTGCAAGTTTGTTGGGTGAATTGGGTCCTTTTGATGTTTCTCCACATTGCC
TCCCATTGCCAGCATTACCCTGA

Last Updated on 06.12.2018 16:11