Sequences from confirmation amplicon


Confirmation sequences for line 395E03 and hit At5g12310 are shown.
Line ID 395E03
Locus At5g12310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3980720
Primers used for PCR 28we - 8474, show primer details
   
Sequence Name 23-K118262-0022-395-E03C-28we
GenBank Accession LN496747 [GenBank]
Sequencing primer gene specific primer, 28we: 5'-CTTGTTTACATTTGGCTCCATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118262-0022-395-E03C-28we
ACTCTTGGCTTTACATCATTTTCTGTACTTCTTCCTTTGTAACTAATTTTGTCTGTTATA
TATCATTGCAGCGTTGAAGATTTCATGTTCGAGGAGAGTGTATGCCTCCTCCTGAGGGCA
TCATGGTTCCAGCCACTGGAAGCAGTGGAGCGGGTTTCAGACAATGACAATTTCAACTAT
GATTTCGACATTCCACCTGAGTATGAGGAGGAGGATGAGGACGACGATGACCTTGACGAG
TTTTATATGCAAGGTTCAAGTCTTCGATTAGGAAACCGGAGGTGGGGTGACAATGGATTT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 03-K118261-0022-395-E03C-8409
GenBank Accession LN487678 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118261-0022-395-E03C-8409
ATTTCAATCCATTGTCACCCCACCTCCGGTTTCCTAATCGAAGACTTGAACCTTGCATAT
AAAACTCGTCAAGGTCATCGTCGTCCTCATCCTCCTCCTCATACTCAGGTGGAATGTCGA
AATCATAGTTGAAATTGTCATTGTCTGAAACCCGCTCCACTGCTTCCAGTGGCTGGAACC
ATGATGCCCTCAGGAGGAGGCATACACTCTCCTCGAACATGAAATCTTCAACGCTGCAAT
GATATATAACAGACAAAATTAGTTACAAAGGAAGAAGTACAGAAAATGATGTAAAAGCCA
AGAGTAAACTAAAGCAATAAAGGCACACCGCCAGTCAAGTAAATGGAGTTTAAAATGGAG
CCAAGGTTAAACAA

Last Updated on 06.12.2018 16:11