Sequences from confirmation amplicon


Confirmation sequences for line 395G01 and hit At1g73190 are shown.
Line ID 395G01
Locus At1g73190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27523038
Primers used for PCR GG29 - 8474, show primer details
   
Sequence Name 95-K100901-0022-395-G01C-8409
GenBank Accession LN515338 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K100901-0022-395-G01C-8409
GCCATTTACAATTGAATATATCAGTGGTCATGCCATCTCCATCCCACCAACGCTGGACCA
AAAGCTCTAGCTGGATTCATCGAAGCACCAGAAAATGGTCCACCTACTAAGATGTTTGCC
CCAACTATGAGTCCGATTGCAAGCGGTGCTATGATCCCGAGGCTTCCACGTTTTGGATCA
ATCAAAGTGGAATACACTACGTAGACTAAGCCAAATGTTAAAATGATCTCTAATACAAGT
CCATTAACCGCTCCAACACCTGATGCTAGACGGAAACCAACTGGTCTCTGCATGAACACA
AACTCTTTTTAAGTCATATACCCTATATTAATATGGTAGGTTCCATCTTAAAAAGCATTG
CAACACTAAGTACTAGCTAGATATATATGAAGTTGACTAGTGTTACCATGCCGTTTGTTG
TGAGCCTTAACAAGAGACAAGCGAGGATGGCTCCNAGAAGCTGAGCGATCCGGTAGTA
   
Sequence Name 95-K100907-0022-395-G01C-GG29
GenBank Accession LN515339 [GenBank]
Sequencing primer gene specific primer, GG29: 5'-CTACTACTGGATCGCTCAGCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K100907-0022-395-G01C-GG29
AGGCTCACAACAACGGCATGGTAACACTAGTCAACTTCATATATATCTAGCTAGTACTTA
GTGTTGCAATGCTTTTTAAGATGGAACCTACCATATTAATATAGGGTATATGACTTAAAA
AGAGTTTGTGTTCATGCAGAGACCAGTTGGTTTCCGTCTAGCATCAGGTGTTGGAGCGGT
TAATGGACTTGTATTAGAGATCATTTTAACATTTGGCTTAGTCTACGTAGTGTATTCCAC
TTTGATTGATCCAAAACGTGGAAGCCTCGGGATCATAGCACCGCTTGCAATCGGACTCAT
AGTTGGGGCAAACATCTTAGTAGGTGGACCATTTTCTGGTGCTTCGATGAATCCAGCTAG
AGCTTTTGGTCCAGCGTTGGTGGGATGGAGATGGCATGACCACTGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA

Last Updated on 06.12.2018 16:11