Sequences from confirmation amplicon


Confirmation sequences for line 395G08 and hit At3g49870 are shown.
Line ID 395G08
Locus At3g49870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18493948
Primers used for PCR JU33 - 8474, show primer details
   
Sequence Name 03-K023618-022-395-G08C-8409
GenBank Accession LN487309 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K023618-022-395-G08C-8409
CATTTACAATTGAAATCGTGTTGCAACCAAATTACATCACCGTTATCAGCTCAATAGTAT
TTGAATCGTGGTCTAGTTTCTTATCGTGGGCTAGTTTTGACAAGGTTCTACAAATAAAGG
CAGATTCTGTATCTTTTAGTTTTTAAAAGAGCAAACTTTAGGCATTTGGTGTGACCTTGT
TTTCTTTTTAGATTGCCAATTTTAGCTATGTGTAAGAATGATAGCATCTTTAATGTGGTT
TATGAAGAAACTATCGGTGTAACATCAATCTAGTTGAAAAGATGCGTTTTTTCAAGGCAT
GTAGAACAGGTTCTAACTTCTAACAATCGTAAAATTTCATGAAAGATTGGTTCTTTTTGG
TATCTAATTTATGTTTCGATTCGGTATTCAATTGTTTGTTTCCATATGATTTTGCAGCCT
CTTCTTCAAGCAAGAAATGGAGCTTTCTTTGATAGGACTTCAAAA
   
Sequence Name 04-K023618-022-395-G08C-JU33
GenBank Accession LN487750 [GenBank]
Sequencing primer gene specific primer, JU33: 5'-CTTTCCTGCATTTTGAAGTCCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K023618-022-395-G08C-JU33
AGCTCCATTTCTTGCTTGAAGAAGAGGCTGCAAAATCATATGGAAACAAACAATTGAATA
CCGAATCGAAACATAAATTAGATACCAAAAAGAACCAATCTTTCATGAAATTTTACGATT
GTTAGAAGTTAGAACCTGTTCTACATGCCTTGAAAAAACGCATCTTTTCAACTAGATTGA
TGTTACACCGATAGTTTCTTCATAAACCACATTAAAGATGCTATCATTCTTACACATAGC
TAAAATTGGCAATCTAAAAAGAAAACAAGGTCACACCAAATGCCTAAAGTTTGCTCTTTT
AAAAACTAAAAGATACAGAATCTGCCTTTATTTGTAGAACCTTGTCAAAACTAGCCCACG
ATAAGAAACTAGACCACGATTCAAATACTATTGAGCTGATAACGGTGATGTAATTTGGTT
GCAACACGATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11