Sequences from confirmation amplicon


Confirmation sequences for line 396C10 and hit At5g67260 are shown.
Line ID 396C10
Locus At5g67260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26837149
Primers used for PCR TK38 - 8474, show primer details
   
Sequence Name 13-K103674-0022-396-C10C-TK38
GenBank Accession LN492006 [GenBank]
Sequencing primer gene specific primer, TK38: 5'-GATGGCCGATAATAATAAGCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K103674-0022-396-C10C-TK38
TTTAAATTTGATCAAGAAAGGTACTAGCGAGGACTATAAGCACATCGAGAAACATACGGT
TTATTGAGGGCAATCTCATTTGCTGCTCCTGAACTCTTCTTCTCTTGAGCAGAGGCTCTG
GAGACGAAGATGATGAGGACACTGAAGCAGTAGTGGAGACATTCCAGGAGCTATTTGAGC
TGTCATCAAAGTCTAATACACCACTTGGACTGTCCTGATCAACCAAATTCATCATCCTCT
TCTTGCTTGGATTGTGCTCCAACAACAGTTCATAGCATTCATTTACTTTCTCCTGTGAAG
TCAATGCTGAATCAGATTCTCATCTTTAACATTATAACTGGACTAATTTACAAAGCCTTT
TTAAGCAACAGACCTGATTGACTTTGAGTAGAGTCGTTATTTGAGATTGGTATTCAACTT
CATCACATGGCTTCAATTCCTCGAAGACAAGGATCATTATTGCAGTAGCTAAGACAGAAG
GGAAGTACCTCATAAACCTCGTATCTGCGACCAAGACTCAGAATTTAAGCAACAAGAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
C
   
Sequence Name 29-K103675-0022-396-C10C-8409
GenBank Accession LN499252 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K103675-0022-396-C10C-8409
CATTTACAATTCTTGTTGCTTAAATTCTGAGTCTTGGTCGCAGATACGAGGTTTATGAGG
TACTTCCCTTCTGTCTTAGCTACTGCAATAATGATCCTTGTCTTCGAGGAATTGAAGCCA
TGTGATGAAGTTGAATACCAATCTCAAATAACGACTCTACTCAAAGTCAATCAGGTCTGT
TGCTTAAAAAGGCTTTGTAAATTAGTCCAGTTATAATGTTAAAGATGAGAATCTGATTCA
GCATTGACTTCACAGGAGAAAGTAAATGAATGCTATGAACTGTTGTTGGAGCACAATCCA
AGCAAGAAGAGGATGATGAATTTGGTTGATCAGGACAGTCCAAGTGGTGTATTAGACTTT
GATGACAGCTCAAATAGCTCCTGGAATGTCTCCACTACTGCTTCAGTGTCCTCATCATCT
TCGTCTCCAGAGCCTCTGCTCAAGAGAAGAAGAGTTCAGGAGCAGCAAATGAGATTGCCC
TCAATAAACCGTATGTTTCTCGATGTGCTTAGTAGTCCTCGCTAGTACCTTTCTTTGATC
AAATGTGTCAAAACATAAATTCAATCTCTCTTTTGCTTATTATTATCGGCCAT

Last Updated on 06.12.2018 16:11