Sequences from confirmation amplicon


Confirmation sequences for line 396E06 and hit At1g23790 are shown.
Line ID 396E06
Locus At1g23790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8411253
Primers used for PCR 20ve - 8474, show primer details
   
Sequence Name 74-K117997-0022-396-E06C-20ve
GenBank Accession LN513144 [GenBank]
Sequencing primer gene specific primer, 20ve: 5'-CACTTGTGCCTTTACATTTCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K117997-0022-396-E06C-20ve
ATTTGCAGCTTCTTTCTCTATCTCTTTACCAACCTCGCCGTGTTTCCTAATCCGGAAAGA
GAAACTGCGGGGTTCTTGTTCTCATCGTTTGATCTCTTTTGCTGCTGCTTCAGGAGAATT
CTTCTCTTTGATGTTGTTTTCTCCTGATCAATAGAGTTATGCTGGATTTCGTGTAGTATT
AATGATCCATCCTCAGATTTTTTCTCAGTAGTAGCAGGTGAAGCAGCTGCTTCGATAGAA
GCAATTTCGTTCATGGTTTCAGATATCTGGCTGTGAAACTCCAAGAACTTGTCGAAGCAA
GCAGCTGGACAATCAGCTTTTGCTGATTTGCTCAGATTTGCAAATGTCCTGCGTTGTCCC
AAATAAGATCCATAAGAACATTTCAAACACAAACATCCATAAATCATACAATGAAACCAA
TCAAGAAGAGATTGTTATGTGTACTTGATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 34-K117996-0022-396-E06C-8409
GenBank Accession LN501585 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117996-0022-396-E06C-8409
TCAATTGAATATCAAGTACACATAACAATCTCTTCTTGATTGGTTTCATTGTATGATTTA
TGGATGTTTGTGTTTGAAATGTTCTTATGGATCTTATTTGGGACAACGCAGGACATTTGC
AAATCTGAGCAAATCAGCAAAAGCTGATTGTCCAGCTGCTTGCTTCGACAAGTTCTTGGA
GTTTCACAGCCAGATATCTGAAACCATGAACGAAATTGCTTCTATCGAAGCAGCTGCTTC
ACCTGCTACTACTGAGAAAAAATCTGAGGATGGATCATTAATACTACACGAAATCCAGCA
TAACTCTATTGATCAGGAGAAAACAACATCAAAGAGAAGAATTCTCCTGAAGCAGCAGCA
AAAGAGATCAAACGATGAGAACAAGAACCCCGCAGTTTCTCTTTCCGGATTAGGAAACAC
GGCGAGGTTGGTTAAAGAGATAGAGAAAGAAGCTGCAAATTGGTTCATGGAGTTTATAGA
AAAGGCTCTGGAGAAAGGGATGAAGAAATGTAAAGGCACAAGTG

Last Updated on 06.12.2018 16:11