Sequences from confirmation amplicon


Confirmation sequences for line 396G05 and hit At4g15230 are shown.
Line ID 396G05
Locus At4g15230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8685515
Primers used for PCR KP52 - 8474, show primer details
   
Sequence Name 55-K034067-022-396-G05C-KP52
GenBank Accession LN508728 [GenBank]
Sequencing primer gene specific primer, KP52: 5'-CCTCCTGGACAGAATTCAAGTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K034067-022-396-G05C-KP52
TTTGGTCTACAATACTCNAGACTAGTTTCCTCTCNGGCTGAGTTTTGGATTTCACAAAAT
AACTTTAAGAGTACTATATATTGACAGAGCTTCTCTGGACTTCCCAAGATCCAAAAGAAT
TGTAATCCAGCCACTTGGATCTTAGATATTACTAGTAAATCTGCAGAGGAAAAACTTGGA
ATAGATTTTTCACAATCATACAAGGATTCAACTCTGTATAAGTGAGTGTTTATGCAACAA
TGCTCTTACTTTTTGCATGTGTGCGTAAAAACCGATTGTTCTCATTGCTGGTTGTTCGGT
CTTTTCTTGCAGGCAGAACAAAATGGTGGTTGAGCAACTGAGTTCTGCGTCTCTGGGATC
AGAAGCTTTGAGGTTTCCTTCGCAATTTTCACAAACAGCTTGGGTGCAACTAAAGGCTTG
CCTTTGGAAACAACATTACTCGTATTGGATCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 39-K034067-022-396-G05C-8409
GenBank Accession LN503269 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K034067-022-396-G05C-8409
TGAAGCCATTTACAATTGAATATATCCTGATCCAATACGAGTAATGTTGTTTCCAAAGGC
AAGCCTTTAGTTGCACCCAAGCTGTTTGTGAAAATTGCGAAGGAAACCTCAAAGCTTCTG
ATCCCAGAGACGCAGAACTCAGTTGCTCAACCACCATTTTGTTCTGCCTGCAAGAAAAGA
CCGAACAACCAGCAATGAGAACAATCGGTTTTTACGCACACATGCAAAAAGTAAGAGCAT
TGTTGCATAAACACTCACTTATACAGAGTTGAATCCTTGTATGATTGTGAAAAATCTATT
CCAAGTTTTTCCTCTGCAGATTTACTAGTAATATCTAAGATCCAAGTGGCTGGATTACAA
TTCTTTTGGATCTTGGGAAGTCCAGAGAAGCTCTGTCAATATATAGTACTCTTAAAGTTA
TTTTGTGAAATCCAAAACTCAGCCGAGAGGAAACTAGTCTAGTATTGTAGACAAACTGAC
CTCGAAGTATTCGATAACCTTACTTGAATTCTGTCCAGGAGGANAGGTGAGACAAACATC
TCATGGCA

Last Updated on 06.12.2018 16:11