Sequences from confirmation amplicon


Confirmation sequences for line 396G05 and hit At4g25320 are shown.
Line ID 396G05
Locus At4g25320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12955256
Primers used for PCR 29qv - 8474, show primer details
   
Sequence Name 33-K115806-0022-396-G05C-29qv
GenBank Accession LN501129 [GenBank]
Sequencing primer gene specific primer, 29qv: 5'-ATGGCTCAAGAAGTCTCAAATGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K115806-0022-396-G05C-29qv
CTTTTAGACCTTCCTTTGATGTCTGTTAAAAGTTAGTAGTGTAACTGACATGGATCTATA
TGTGGGGTTGACTTGTACGATGTTGGAATAAGAAGAGAACTTTTTGTGTCAGAAGTCATA
AGCTAGTGAAGAGAAAGGCTACGACTTTAGGGGAAGTAGATTAGAATATGCACATCACAA
CATTTCCCAAAAGCTCAGGGATTAGGAGAGAACAGCTTTAATTAAGTACAGTCTTGGTCT
TGTGTTTGTCTTATAGATCAGTTACATCTTGTTTTAGTTTGATATTTGTCTGTCTTGTGT
ATATCTTAGTGTTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCA
   
Sequence Name 12-K115805-0022-396-G05C-8409
GenBank Accession LN491725 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K115805-0022-396-G05C-8409
ATTTCAAACACTAAGATATACACAGACAGACAAATATCAAACTAAAACAAGATGTAACTG
ATCTATAAGACAAACACAAGACCAAGACTGTACTTAATTAAAGCTGTTCTCTCCTAATCC
CTGAGCTTTTGGGAAATGTTGTGATGTGCATATTCTAATCTACTTCCCCTAAAGTCGTAG
CCTTTCTCTTCACTAGCTTATGACTTCTGACACAAAAAGTTCTCTTCTTATTCCAACATC
GTACAAGTCAACCCCACATATAGATCCATGTCAGTTACACTACTAACTTTTAACAGACAT
CAAAGGAAGGTTCTAAAAAGATATTTACCAGGACTTCTATCGAATTGGAACATTTGAGAC
TTCNTGAGCCA

Last Updated on 06.12.2018 16:11