Sequences from confirmation amplicon


Confirmation sequences for line 396H05 and hit At2g23140 are shown.
Line ID 396H05
Locus At2g23140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9845738
Primers used for PCR 07ht - 8474, show primer details
   
Sequence Name 37-K112586-0022-396-H05C-07ht
GenBank Accession LN502670 [GenBank]
Sequencing primer gene specific primer, 07ht: 5'-ATCTTATTGATCTGATGGACCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K112586-0022-396-H05C-07ht
TTTTGGCAATCTGCTCAATTCCGGAAGGAAAAACGCGATTGGTCAAGAAGGCGGAATCCC
TCTTCTTGTTGAAGTCGTTGAGTTGGGTTCAGCTAGAGGGAAAGAAAACGCAGCAGCAGC
TCTTCTTCAACTTTCAACCAACAGTGGTCGGTTCTGCAACATGGTTCTTCAAGAAGGCGC
CGTTCCTCCACTCGTCGCTCTCTCACAGTCTGGTACTCCTAGAGCTAGAGAAAAGGTACA
AACTTTATAACTAATATATCATGTTCCTCTTAATTTAAGTAGCCACACTCATGACTTTGA
TTGTTTTGAAGAGTTCTTTATTTGGAGAAAATCTTGTAACTTGTCAGGCACAGGCGTTGC
TCAGTTACTTCAGAAACCATGCCGTTGGAGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCACAGTTATTA
   
Sequence Name 11-K112585-0022-396-H05C-8409
GenBank Accession LN491185 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K112585-0022-396-H05C-8409
CTGAGTACTGAGCACGCCTGTGCCTGACAANTTACAAGATTTTCTCCAAATAAAGAACTC
TTCAAAACAATCAAAGTCATGAGTGTGGCTACTTAAATTAAGAGGAACATGATATATTAG
TTATAAAGTTTGTACCTTTTCTCTAGCTCTAGGAGTACCAGACTGTGAGAGAGCGACGAG
TGGAGGAACGGCGCCTTCTTGAAGAACCATGTTGCAGAACCGACCACTGTTGGTTGAAAG
TTGAAGAAGAGCTGCTGCTGCGTTTTCTTTCCCTCTAGCTGAACCCAACTCAACGACTTC
AACAAGAAGAGGGATTCCGCCTTCTTGACCAATCGCGTTTCTTCCTTCCGGAATTGTAGC
TAGATTTGCCAAAACAGCAACTGCTTTATCCACCATCCCAGCTGCTGGGTCCATCAGACA
AATAAGA

Last Updated on 06.12.2018 16:11