Sequences from confirmation amplicon


Confirmation sequences for line 397B05 and hit At1g58310 are shown.
Line ID 397B05
Locus At1g58310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21633130
Primers used for PCR CB48 - 8474, show primer details
   
Sequence Name 11-K106136-0022-397-B05C-8409
GenBank Accession LN491121 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K106136-0022-397-B05C-8409
CGGACTGAGCCATTTCAATTGTCCGTGTCGGGCCTTCTCAGAATTCTCAAATCTGGAAAC
TCTTGTCTTTAAAGTATGAAACAAACCCTTTCAAGCATTTGTTGACATATTTTTCAAAGT
TTGAGATGTTTCTCCCACATTTCAGGGACTTGTCCACCGTTTCACGGATAAATGTGGAGA
TATGTGTTTATGCAAACCTTGGGAGGAGGATGAAGAAGAGGAGGAAGACGAGGAGACTCC
TACTTGCTTATCATCATGTCCAGTGAAAGTGCTGAAGATATTGGAATTTGGTAAAATCTA
TGATGACGAGATAGAGAAGAGGATAGACCAAGTCAAGCATTTTCTAGAGAAGATACCGGA
AATTGAGC
   
Sequence Name 11-K106135-0022-397-B05C-CB48
GenBank Accession LN491120 [GenBank]
Sequencing primer gene specific primer, CB48: 5'-TGCTCAATATCCGGTATCTTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K106135-0022-397-B05C-CB48
TCTTCTCATCTCGTCATCATAGATTTTACCAAATTCCAATATCTTCAGCACTTTCACTGG
ACATGATGATAAGCAAGTAGGAGTCTCCTCGTCTTCCTCCTCTTCTTCATCCTCCTCCCA
AGGTTTGCATAAACACATATCTCCACATTTATCCGTGAAACGGTGGACAAGTCCCTGAAA
TGTGGGAGAAACATCTCAAACTTTGAAAAATATGTCAACAAATGCTTGAAAGGGTTTGTT
TCATACTTTAAAGACAAGAGTTTCCAGATTTGGAGAATTCTTGAGAAGGCCCGACACGGA
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
C

Last Updated on 06.12.2018 16:11