Sequences from confirmation amplicon


Confirmation sequences for line 397C02 and hit At5g07390 are shown.
Line ID 397C02
Locus At5g07390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2339072
Primers used for PCR SS07 - 8474, show primer details
   
Sequence Name 07-K026133-022-397-C02C-8409
GenBank Accession LN489108 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K026133-022-397-C02C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGCTTTAGCGAGGAGGGAAAACGTAAGC
GGAGATTCAATAAACATTAATGAGCTTAAAGAGTTCTGGAAGCAGATCACTGATCAAGAT
TTTGATTCAAGGCTACGAACTTTCTTCGCCATGTAAATGTTTCTTTCGATTTCTCTATAT
ATTTGACATGAACTTTGAGTTTTATATTGAAGTGTGGTATGAAATTTAGGGTCGATAAGG
ATTCGGATGGGCGGTTGAATGAAGCCGAAGTAAGAGAGGTAGAATCTTGATTTTTGTAGT
GATTAGTATTAACAATCTTGCAGCTCTTTGTGTTCTTCATCATAAGTATATTTTCTATAT
GTGTATAGATTATAACTTTAAGTGCTTCTGCAAACGAGCTGGATAACATTCGGAGACAAG
CTGATGAATATGCTGCTTTAATTATGGAAGAACTAGATCCTTATCATTATGGGTACATCA
TGGTATGTGAAGTTTTGTGAATGAGGTTTGTTTTGATTTAATCATTTTTTTAGTTAGAAA
AGTTTGGTTTGTGAGTGTTTTTTGATGTGTTTGGAGGCACTTGCTACAATGCCAAACAGA
TAGAGAATCTCGAGATACTTCTATTGCAAGCG
   
Sequence Name 08-K026133-022-397-C02C-SS07
GenBank Accession LN489572 [GenBank]
Sequencing primer gene specific primer, SS07: 5'-CTTACCCTCTCCATCTCTCACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K026133-022-397-C02C-SS07
CTGCATCGGCGCTTGCAATAGAAGTATCTCGAGATTCTCTATCTGTTTGGCATTGTAGCA
AGTGCCTCCAAACACATCAAAAAACACTCACAAACCAAACTTTTCTAACTAAAAAAATGA
TTAAATCAAAACAAACCTCATTCACAAAACTTCACATACCATGATGTACCCATAATGATA
AGGATCTAGTTCTTCCATAATTAAAGCAGCATATTCATCAGCTTGTCTCCGAATGTTATC
CAGCTCGTTTGCAGAAGCACTTAAAGTTATAATCTATACACATATAGAAAATATACTTAT
GATGAAGAACACAAAGAGCTGCAAGATTGTTAATACTAATCACTACAAAAATCAAGATTC
TACCTCTCTTACTTCGGCTTCATTCAACCGCCCATCCGAATCCTTATCGACCCTAAATTT
CATACCACACTTCAATATAAAACTCAAAGTTCATGTCAAATATATAGAGAAATCGAAAGA
AACATTTACATGGCGAAGAAAGTTCGTAGCCTTGAATCAAAATCCTTGATCAGTGATCTG
CTTCCAGAACTCTTTAAGCTCATTAATGTTTATTGAATCTCCGCTTACGTTTTCCCTCCT
CGCTAAAGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGAATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
G

Last Updated on 06.12.2018 16:11