Sequences from confirmation amplicon


Confirmation sequences for line 397H07 and hit At1g24330 are shown.
Line ID 397H07
Locus At1g24330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8634194
Primers used for PCR 58rq - 8474, show primer details
   
Sequence Name 21-K116018-0022-397-H07C-58rq
GenBank Accession LN495804 [GenBank]
Sequencing primer gene specific primer, 58rq: 5'-TTGAATCTGAACGACAACAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116018-0022-397-H07C-58rq
ATTCTGAATCAATGTTCTAGAGGGGTATCAGGATATATTGGCAATCGTGGACAAAGAGGA
GGACTTAGCTAAAAAGTGCAAAGTAGTAGAAAATGTCAGGATATTGCTAAAGGATAATGA
AGAGGCCAGGATCCTCATGGGAGCCAATGGGTTCGTGGAAGCGTTTTTGCAGTTTCTAGA
ATCAGCTGTTCATGATAACAATGCAGCCGCACAGGAAACCGGAGCTATGGCTCTATTTAA
CTTAGCAGTCAACAATAACAGGTTAGTTATTATCACATCCTTCCTGTCTTTGTTACCAAT
CAAATTCCTTAGCAATGTCACTTTCTAACATCCCATTCATGGTGCTCTTCATTTGCAAAC
CTTGTCATAATCTCTTCTGTTTCGTTCTTTTGGATGCACTGCAGGAACAAAGAGCTGATG
CTGACTTCAGGGGTCATCCCACTGCTAGAGAAAATGATTTCTTGTTCCCAATCCCAAGGG
CCAGCAACTGCGTTATATCTAAACCTCTCTTGCCTCGAGAAAGCCAAGCCCGTGATTGGC
TCAAGTCAAGCAGTTTCCTTTTTCGTGAATCTTCTTCTTCAGGATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAA
   
Sequence Name 08-K116017-0022-397-H07C-8409
GenBank Accession LN489879 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K116017-0022-397-H07C-8409
GATTCACGAAAAAGGAAACTGCTTGACTTGAGCCAATCACGGGCTTGGCTTTCTCGAGGC
AAGAGAGGTTTAGATATAACGCAGTTGCTGGCCCTTGGGATTGGGAACAAGAAATCATTT
TCTCTAGCAGTGGGATGACCCCTGAAGTCAGCATCAGCTCTTTGTTCCTGCAGTGCATCC
AAAAGAACGAAACAGAAGAGATTATGACAAGGTTTGCAAATGAAGAGCACCATGAATGGG
ATGTTAGAAAGTGACATTGCTAAGGAATTTGATTGGTAACAAAGACAGGAAGGATGTGAT
AATAACTAACCTGTTATTGTTGACTGCTAAGTTAAATAGAGCCATAGCTCCGGTTTCCTG
TGCGGCTGCATTGTTATCATGAACAGCTGATTCTAGAAACTGCAAAAACGCTTCCACGAA
CCCATTGGCTCCCATGAGGATCCTGGCCTCTTCATTATCCTTTAGCAATATCCTGACATT
TTCTACTACTTTGCACTTTTTAGCTAAGTCCTCCTCTTTGTCCACGATTGCCAATATATC
CTGATACCCCTCTAGAACATTGATTTCAGAATCCACCTCATCAGGAGCATTGTTTTTTTC
TTTTTGTTGTC

Last Updated on 06.12.2018 16:11