Sequences from confirmation amplicon


Confirmation sequences for line 399C04 and hit At1g60930 are shown.
Line ID 399C04
Locus At1g60930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22435094
Primers used for PCR HF13 - 8474, show primer details
   
Sequence Name 46-K032417-022-399-C04C-8409
GenBank Accession LN505769 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K032417-022-399-C04C-8409
TTGAATATATCCTGCTCTACTCTGTGCAGGAATAACATTAGTCATTTCTCCACTTGTTTC
ACTCATTCAAGACCAGATAATGAACTTATTGCAGGTGAGAACTTTAGCAACAGAATGTTG
TTTCTGTCTTTAGTTTCATTTCTATGTTGTACTCAGAAAATTTGTCCCGATTAACACTTT
GGTTTCTGAAACATGGCTATAGACCAATATATCTGCTGCTTCCTTAAGTGCCGGAATGGA
ATGGGCTGAACAACTAGAGATACTTCAGGAGCTGAGCTCTGAGAAGTCTAAATACAAGTT
ACTGTATGTAACACCAGAAAAAGTGGCAAAGTGAGTCATTATTTTCTTTAGTTGTTGATT
CTGAGAAGAATGTGGGTATTCAAAAAATGCCCATCGGATAGGAGTGG
   
Sequence Name 46-K032417-022-399-C04C-HF13
GenBank Accession LN505770 [GenBank]
Sequencing primer gene specific primer, HF13: 5'-TTTAATTTGTTCCACTCCTATCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K032417-022-399-C04C-HF13
TCTTCTCGAATCAACAACTAAAGAAAATAATGACTCACTTTGCCACTTTTTCTGGTGTTA
CATACAGTAACTTGTATTTAGACTTCTCAGAGCTCAGCTCCTGAAGTATCTCTAGTTGTT
CAGCCCATTCCATTCCGGCACTTAAGGAAGCAGCAGATATATTGGTCTATAGCCATGTTT
CAGAAACCAAAGTGTTAATCGGGACAAATTTTCTGAGTACAACATAGAAATGAAACTAAA
GACAGAAACAACATTCTGTTGCTAAAGTTCTCACCTGCAATAAGTTCATTATCTGGTCTT
GAATGAGTGAAACAAGTGGAGAAATGACTAATGTTATTCCTGCACAGAGTAGAGCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACTTC

Last Updated on 06.12.2018 16:11