Genotyping and primer details


Genotyping and primer details for 399C04 - At1g60930
Line ID 399C04
Locus At1g60930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22435094 of line 399C04 Chr1:22435094
   
Insertion allele amplicon 8474 - HF13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HF13: 5'-TTTAATTTGTTCCACTCCTATCCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22434687
   
wt amplicon (over InsPos) 36rd - HF13
wt amplicon start Chr1:22434687
wt amplicon end Chr1:22435552
wt amplicon size 866 bp
Locus specific primer HF13: 5'-TTTAATTTGTTCCACTCCTATCCG-3'
2nd locus specific primer 36rd: 5'-GAAAAATCTGGAGGTGATTGGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11