Sequences from confirmation amplicon


Confirmation sequences for line 399C06 and hit At2g20620 are shown.
Line ID 399C06
Locus At2g20620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8892970
Primers used for PCR CB52 - 8474, show primer details
   
Sequence Name 05-K105704-0022-399-C06C-CB52
GenBank Accession LN488351 [GenBank]
Sequencing primer gene specific primer, CB52: 5'-AGTTATACCTTCAAGGCTGCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K105704-0022-399-C06C-CB52
CTTACGGCAGCATCCCAAAGGCATATTTCATACTGGAAAGGGAATTGTTGGCTGGATCAA
AGGCATCCAATATCATATGATAATTGATATGTGCAACCGATTGCAGCATGACTTGGGTTA
CAGTCATCAACTCTAAATTTGTCCCCTACAATGATAATAACCGTAAGTGGAGATGTAACA
GTTAAAATCCGAAGAAGAAGAAGAAGTAAAGGTGAGGGCTTGCTAGAATAAGAGCAATAT
AGATAAGAGTTACCGTTTGAGAATTGTAGGAATGGAGTGCCACCTCTGCAAGGAGTTCGT
CCGGGAAATATGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 21-K105705-0022-399-C06C-8409
GenBank Accession LN495659 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K105705-0022-399-C06C-8409
TTCCGGACGACTCCTTGCGAGGTGGCACTCCATTCCTACATTCTCAAACGGTAACTCTTA
TCTATATTGCTCTTATTCTAGCAAGCCCTCACCTTTACTTCTTCTTCTTCTTCGGATTTT
AACTGTTACATCTCCACTTACGGTTATTATCATTGTAGGGGACAAATTTAGAGTTGATGA
CTGTAACCCAAGTCATGCTGCAATCGGTTGCACATATCAATTATCATATGATATTGGATG
CCTTTGATCCAGCCAACAATTCCCTTTCCAGTATTGAAATATGCCTTTGGGATGCTGCCG
TAAAGAATAATGAGAAATTGAGATTAGTCACCACTTTTTGCAGCCTTGAAGGGTATAAC

Last Updated on 06.12.2018 16:11