Sequences from confirmation amplicon


Confirmation sequences for line 399G04 and hit At4g33320 are shown.
Line ID 399G04
Locus At4g33320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16057067
Primers used for PCR 16wz - 8474, show primer details
   
Sequence Name 35-K118924-0022-399-G04C-16wz
GenBank Accession LN502017 [GenBank]
Sequencing primer gene specific primer, 16wz: 5'-AGAAGATTGAGGAAGCAAACAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K118924-0022-399-G04C-16wz
TTAGTTAGAGGATGTCTCAAACGAAGGTTCTGGTGGAGATGGAAATTTACACTCTTCCTG
ACTTGACTACTGAACTCTTTGTATCTACTTACGAAGTTGCTGCTAAAGCTGTGCATGATT
TCTCCAAGCCGCTAATCAACATGATGAAAGCAGCAGGATGGGATCTTGATTCTGCAGCCA
ATTCTATTGAGCCTGATGTTGTTTACGCCAAGAGGCCTCACAAGAAATATGCATTTGAAT
CATACATATGCCAAAGGATGTTCAGTGGGTTTCAGCAGAAGAACTTCTCAGTAAACTCAG
AGAGTGCTACGGTTATGGCCGATGATGACACAGACACCTTTTTCCGCCAGTTTCTTGCTC
TCAAGGACATGGATCCACTAGATGCTCTAGGTACAAACCCTGATTCCAACGTTCGTTTAT
ATGCTTGTCAAAAAGTTTTTGTAATTGTTGGGAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 11-K118923-0022-399-G04C-8409
GenBank Accession LN491344 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118923-0022-399-G04C-8409
AAACTTTTTGACAGCATATAAACGAACGTTGGAATCAGGGTTTGTACCTAGAGCATCTAG
TGGATCCATGTCCTTGAGAGCAAGAAACTGGCGGAAAAAGGTGTCTGTGTCATCATCGGC
CATAACCGTAGCACTCTCTGAGTTTACTGAGAAGTTCTTCTGCTGAAACCCACTGAACAT
CCTTTGGCATATGTATGATTCAAATGCATATTTCTTGTGAGGCCTCTTGGCGTAAACAAC
ATCAGGCTCAATAGAATTGGCTGCAGAATCAAGATCCCATCCTGCTGCTTTCATCATGTT
GATTAGCGGCTTGGAGAAATCATGCACAGCTTTAGCAGCAACTTCGTAAGTAGATACAAA
GAGTTCAGTAGTCAAGTCAGGAAACTGTAAATTTCCATCTCCACCAGAACCTTCGTTTGT
AGACATTCCTCTTAACTTAAGATTCTTCTCAAGCTTAAGCCGTTTCTTGTTTGCTTCCTC
AATCTTC

Last Updated on 06.12.2018 16:11